| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GPR43 |
| Specificity: |
This antibody reacts specifically with GPR43 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GPR43 |
| Gene ID: |
2867 |
| Swiss Prot: |
O15552 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Finally, G(i/o)-alpha family of G proteins but also to the Gq family (PubMed:12496283, In addition to SCFAs, In omnivorous mammals, In parallel to its role in energy homeostasis, Its activation results in the formation of inositol 1, May also play a role in the resolution of the inflammatory response and control chemotaxis in neutrophils, May also regulate the production of LEP/Leptin, May play a role in glucose homeostasis by regulating the secretion of GLP-1, PubMed:12711604, PubMed:23589301), SCFAs serve as a source of energy but also act as signaling molecules, That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, a hormone acting on the central nervous system to inhibit food intake, and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity, in response to short-chain fatty acids accumulating in the intestine, may also be activated by the extracellular lectin FCN1 in a process leading to activation of monocytes and inducing the secretion of interleukin-8/IL-8 in response to the presence of microbes (PubMed:21037097), may also mediate the activation of the inflammatory and immune responses by SCFA in the intestine, may also regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers, regulating the rapid production of chemokines and cytokines, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation, the short chain fatty acids (SCFAs), the short chain fatty acids acetate, 4, 5-trisphosphate, G protein-coupled receptor that is activated by a major product of dietary fiber digestion |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FFA2R |
| Also known as: |
GPR43 Polyclonal Antibody |
| Other name: |
Anti-GPR43 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GPR43 |
| Short name: |
Anti-GPR43 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GPR43 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4501 |
| Gene: |
FFAR2 |
More about : FFAR2 |
| Long gene name: |
free fatty acid receptor 2 |
| Synonyms gene: |
GPR43 |
| Synonyms gene name: |
G protein-coupled receptor 43 |
| Locus: |
19q13, 12 |
| Discovery year: |
1997-10-10 |
| GenBank acession: |
AF024690 |
| Entrez gene record: |
2867 |
| Pubmed identfication: |
9344866 |
| RefSeq identity: |
NM_005306 |
| Classification: |
Free fatty acid receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000183303 |