| Antigen detectable with this ELISA kit: |
Ciliary Neurotrophic Factor (CNTF) |
| Species reactivity: |
Porcine (Pig), Sus scrofa |
| Range of detection: |
1000pg/mL, 125pg/mL, 2000pg/mL, 250pg/mL, 500pg/mL, 62, 5-4000pg/mL The standard curve concentrations used for the ELISA&rsquo, 5pg/mL, 62, s were 4000pg/mL |
| Sensitivity: |
4pg/mL, The minimum detectable dose of this kit is typically less than 22 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ciliary Neurotrophic Factor (CNTF), The concentration of Ciliary Neurotrophic Factor (CNTF) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ciliary Neurotrophic Factor (CNTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Ciliary Neurotrophic Factor (CNTF), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Ciliary Neurotrophic Factor (CNTF) |
| Short name: |
Ciliary Neurotrophic Factor (CNTF) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Ciliary Neurotrophic Factor (ciliary neurotrophic factor) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CNTF and IDBG-409278 and ENSG00000242689 and 1270, CNTF and IDBG-637978 and ENSBTAG00000003624 and 100848228, Cntf and IDBG-254935 and ENSMUSG00000079415 and 12803, Extracellular, HCNTF, growth factor activity, this GO :0005127 and ciliary neurotrophic factor receptor binding and molecular function this GO :0005138 and interleukin-6 receptor binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0007165 and signal transduction and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0040007 and growth and biological process this GO :0042517 and positive regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0046533 and negative regulation of photoreceptor cell differentiation and biological process this GO :0046668 and regulation of retinal cell programmed cell death and biological process this GO :0048644 and muscle organ morphogenesis and biological process this GO :0048666 and neuron development and biological process this GO :0070120 and ciliary neurotrophic factor-mediated signaling pathway and biological process this GO :0097058 and CRLF-CLCF1 complex and cellular component, this GO :0005127 : ciliary neurotrophic factor receptor binding, this GO :0005127 : ciliary neurotrophic factor receptor binding and also this GO :0005138 : interleukin-6 receptor binding and also this GO :0008083 : growth factor activity, this GO :0005138 : interleukin-6 receptor binding, this GO :0008083 : growth factor activity, ciliary neurotrophic factor |
| Identity: |
2169 |
| Gene: |
CNTF |
More about : CNTF |
| Long gene name: |
ciliary neurotrophic factor |
| Synonyms: |
HCNTF |
| Locus: |
11q12, 1 |
| Discovery year: |
1991-01-07 |
| GenBank acession: |
BC068030 |
| Entrez gene record: |
1270 |
| Pubmed identfication: |
1840538 1714745 |
| RefSeq identity: |
NM_000614 |
| Classification: |
Interleukin 6 cytokine family |
| Havana BLAST/BLAT: |
OTTHUMG00000137476 |
| Locus Specific Databases: |
ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database |