| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GJA3/Connexin 46 |
| Specificity: |
This antibody reacts specifically with GJA3/Connexin 46 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GJA3/Connexin 46 |
| Gene ID: |
2700 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Connexin proteins share a common topology of four transmembrane Î, Consequently, Each of the approximately 20 connexin isoforms produces channels with distinct permeabilities and electrical and chemical sensitivities, For instance, Many of the key functional differences arise from specific amino acid substitutions in the most highly conserved domains, a cytoplasmic loop and cytoplasmic N- and C-termini, a wide variety of malignant phenotypes associate with decreased connexin expression and gap junction communication, deletion of the gene encoding connexin 46, dependent on the particular connexin that is affected, normally expressed in the lens, one connexin usually cannot fully substitute for another, produces severe cataracts, the transmembrane and extracellular regions, therefore, two extracellular loops, &euro, &euro, &oelig, ±, -helical domains, The connexin family of proteins form hexameric complexes called â, connexonsâ, that facilitate movement of low molecular weight proteins between cells via gap junctions |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CX46 |
| Also known as: |
GJA3/Connexin 46 Polyclonal Antibody |
| Other name: |
Anti-GJA3/Connexin 46 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GJA3/Connexin 46 |
| Short name: |
Anti-GJA3/Connexin 46 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GJA3/Connexin 46 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4277 |
| Gene: |
GJA3 |
More about : GJA3 |
| Long gene name: |
gap junction protein alpha 3 |
| Synonyms gene: |
CZP3 |
| Synonyms gene name: |
46kD (connexin 46) gap junction protein, 46kDa , 46kDa (connexin 46) gap junction protein, alpha 3, alpha 3, alpha 3, gap junction protein |
| Synonyms name: |
connexin 46 |
| Locus: |
13q12, 11 |
| Discovery year: |
1990-02-12 |
| GenBank acession: |
AF075290 |
| Entrez gene record: |
2700 |
| Pubmed identfication: |
10205266 7342922 |
| RefSeq identity: |
NM_021954 |
| Classification: |
Gap junction proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000016510 |