| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
FAU/MNSF-beta |
| Specificity: |
This antibody reacts specifically with FAU/MNSF-beta |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human FAU/MNSF-beta |
| Gene ID: |
2197 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A wide range of enzymes facilitate in the proteolytic Ub pathway, MNSF-beta is a ubiquitin-like fusion protein consisting of the ribosomal protein S30 and a protein that shares 36% sequence identity with ubiquitin, The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex, The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate, The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component, This ubiquitin-like segment (Ubi-L) can be cleaved from MNSF-beta in the cytosol, a subunit of MNSF, including monoclonal nonspecific suppressor factor-beta (MNSF-beta), which is a lymphokine product of a murine T cell hybrid-oma that restricts the production of LPS-induced immunoglobulin secreting cells in an antigen-nonspecific manner, The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RPS30 FLJ22986 Fub1 Fubi MNSFbeta asr1 S30 |
| Also known as: |
FAU/MNSF-beta Polyclonal Antibody |
| Other name: |
Anti-FAU/MNSF-beta Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
FAU/MNSF-beta |
| Short name: |
Anti-FAU/MNSF-beta (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-FAU/MNSF-b (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3597 |
| Gene: |
FAU |
More about : FAU |
| Long gene name: |
FAU, ubiquitin like and ribosomal protein S30 fusion |
| Synonyms gene name: |
Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derived) Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed |
| Synonyms name: |
ribosomal protein S30 Monoclonal nonspecific suppressor factor beta |
| Locus: |
11q13, 1 |
| Discovery year: |
1992-12-02 |
| GenBank acession: |
AK026639 |
| Entrez gene record: |
2197 |
| Pubmed identfication: |
8406491 |
| RefSeq identity: |
NM_001997 |
| Classification: |
S ribosomal proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000165606 |