| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
EYA4 |
| Specificity: |
This antibody reacts specifically with EYA4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human EYA4 |
| Gene ID: |
2070 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A 271 amino acid domain at the carboxyl terminal is highly conserved amongst the members of the eyes absent family (1), DACH, EYA is one of four members of the eyes absent family (1), EYA4 (eyes absent) (1), EYA4 is expressed in the craniofacial mesenchyme, EYA4 may cause oculo-dento-digital syndrome, Premature stop codon mutations in EYA4 cause postlingual, SIX translocates EYA1-3 to the nucleus, The conserved region in other EYA proteins interacts with SIX, This shows that EYA4 is also vital to the mature organ of Corti (2), and G-proteins, and G-proteins can stop this interaction (3, and the limb (1), based on its expression pattern and map postion (1), progressive autosomal dominant hearing loss in humans (2), the dermamyotome, which regulate transcription in early embryonic development (1, 2, 3, 4), 4), A gene of chromosome 6q23 encodes the 640 amino acid protein |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
EYA4 Polyclonal Antibody |
| Other name: |
Anti-EYA4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
EYA4 |
| Short name: |
Anti-EYA4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-EYA4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3522 |
| Gene: |
EYA4 |
More about : EYA4 |
| Long gene name: |
EYA transcriptional coactivator and phosphatase 4 |
| Synonyms gene: |
DFNA10 CMD1J |
| Synonyms gene name: |
eyes absent (Drosophila) homolog 4 eyes absent homolog 4 (Drosophila) |
| Locus: |
6q23, 2 |
| Discovery year: |
1998-07-15 |
| GenBank acession: |
Y17114 |
| Entrez gene record: |
2070 |
| Pubmed identfication: |
9887327 11159937 |
| RefSeq identity: |
NM_004100 |
| Classification: |
EYA transcriptional coactivator and phosphatases |
| Havana BLAST/BLAT: |
OTTHUMG00000015602 |
| Locus Specific Databases: |
Hereditary Hearing Loss Homepage LRG_418 |