| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ERCC8/CSA |
| Specificity: |
This antibody reacts specifically with ERCC8/CSA |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human ERCC8/CSA |
| Gene ID: |
1161 |
| Swiss Prot: |
Q13216 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes, It is required for the recruitment of XAB2, The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair, Substrate-recognition component of the CSA complex |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CSA |
| Also known as: |
ERCC8/CSA Polyclonal Antibody |
| Other name: |
Anti-ERCC8/CSA Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ERCC8/CSA |
| Short name: |
Anti-ERCC8/CSA (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, complementation family 8/CSA (polyclonal), antibody to-excision repair cross-complementing rodent repair deficiency |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
CKN1 and CSA and UVSS2, ERCC8 and IDBG-22959 and ENSG00000049167 and 1161, ERCC8 and IDBG-628902 and ENSBTAG00000021606 and 518857, Ercc8 and IDBG-181454 and ENSMUSG00000021694 and 71991, complementation group 8, nuclei, protein complex binding, this GO :0000109 and nucleotide-excision repair complex and cellular component this GO :0000209 and protein polyubiquitination and biological process this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0006281 and DNA repair and biological process this GO :0006283 and transcription-coupled nucleotide-excision repair and biological process this GO :0006289 and nucleotide-excision repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0009411 and response to UV and biological process this GO :0010165 and response to X-ray and biological process this GO :0016363 and nuclear matrix and cellular component this GO :0031464 and Cul4A-RING E3 ubiquitin ligase complex and cellular component this GO :0032403 and protein complex binding and molecular function this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0043234 and protein complex and cellular component this GO :0045739 and positive regulation of DNA repair and biological process this GO :0051865 and protein autoubiquitination and biological process, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0032403 : protein complex binding, this GO :0005515 : protein binding, this GO :0032403 : protein complex binding, excision repair cross-complementing rodent repair deficiency |
| Identity: |
3439 |
| Gene: |
ERCC8 |
More about : ERCC8 |
| Long gene name: |
CSA ubiquitin ligase complex subunit , ERCC excision repair 8 |
| Synonyms gene: |
CKN1 |
| Synonyms gene name: |
Cockayne syndrome 1 (classical) excision repair cross-complementing rodent repair deficiency, complementation group 8 excision repair cross-complementation group 8 |
| Locus: |
5q12, 1 |
| Discovery year: |
1995-02-07 |
| GenBank acession: |
U28413 |
| Entrez gene record: |
1161 |
| Pubmed identfication: |
8596535 |
| RefSeq identity: |
NM_000082 |
| Classification: |
ERCC excision repair associated WD repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000097741 |
| Locus Specific Databases: |
LRG_466 |