| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ASAH1 |
| Specificity: |
This antibody reacts specifically with ASAH1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Acid ceramidase subunit beta |
| Gene ID: |
427 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Bioinformatic analysis of gene expression also reveals acid ceramidase to be among the 5 most important genes associated with melanoma, Effected individuals experience early onset joint problems and neurological problems, In addition to ceramide hydrolysis, Interestingly, a hallmark of Farber disease, and deficiency leads to accumulation of ceramide in tissues, for hydrolysis the pH optimum is 4, further supporting a complex and central role for acid ceramidase in sphingolipid metabolism, owing to mutations in the acid ceramidase gene, pH regulates which reaction is favored, purified acid ceramidase also exhibits the ability to catalyze ceramide synthesis, utilizing [14C]lauric acid and sphingosine as substrates, whereas for the reverse reaction favors a pH of 5, 5, 5, Acid ceramidase catalyzes the degradation of ceramide in normal tissues |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
AC PHP32 FLJ21558 ACDase |
| Also known as: |
ASAH1 Polyclonal Antibody |
| Other name: |
Anti-ASAH1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ASAH1 |
| Short name: |
Anti-ASAH1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ASAH1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
735 |
| Gene: |
ASAH1 |
More about : ASAH1 |
| Long gene name: |
N-acylsphingosine amidohydrolase 1 |
| Synonyms gene: |
ASAH |
| Synonyms gene name: |
N-acylsphingosine amidohydrolase (acid ceramidase) |
| Synonyms name: |
acylsphingosine deacylase acid ceramidase |
| Locus: |
8p22 |
| Discovery year: |
1997-08-22 |
| GenBank acession: |
U70063 |
| Entrez gene record: |
427 |
| Pubmed identfication: |
8955159 10610716 |
| RefSeq identity: |
NM_004315 |
| Havana BLAST/BLAT: |
OTTHUMG00000096997 |