| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SP6 |
| Specificity: |
This antibody reacts specifically with SP6 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SP6 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
EPIPROFIN or KLF14, Expressed ubiquitously with higher expression in developing teeth, Human Sp6 shares 96% sequence homology with its mouse counterpart, Sp6, Sp6 functions to bind GC-rich sequences and related GT and CACCC boxes, The gene encoding Sp6 maps to human chromosome 17, also known as EPFN, hair follicles and limb buds, is a 376 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers, most of which encompass some form of transcriptional activation or repression, suggesting a conserved role between species, thereby promoting cellular proliferation, which comprises over 2, 200 genes, 5% of the human genome and encodes over 1, Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KLF14 Epfn |
| Also known as: |
SP6 Polyclonal Antibody |
| Other name: |
Anti-SP6 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SP6 |
| Short name: |
Anti-SP6 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SP6 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
14530 |
| Gene: |
SP6 |
More about : SP6 |
| Long gene name: |
Sp6 transcription factor |
| Synonyms name: |
epiprofin |
| Locus: |
17q21, 32 |
| Discovery year: |
2001-02-06 |
| Entrez gene record: |
80320 |
| Pubmed identfication: |
11087666 14551215 |
| RefSeq identity: |
NM_199262 |
| Classification: |
Sp transcription factors Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000132067 |