| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Manic Fringe/MFNG |
| Specificity: |
This antibody reacts specifically with Manic Fringe/MFNG |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Manic Fringe/MFNG |
| Gene ID: |
4242 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Each mammalian fringe displays different patterns of expression, Fringe proteins act upstream of the Notch signaling pathway and are involved in boundary determination during segmentation, Lunatic fringe is required for normal somite segmentation and patterning and is thought to be a target of the molecular clock, Manic, Manic fringe, Radical and Lunatic Fringe, Radical fringe plays a key role in the development of the limb bud, a protein involved in development, also involved in somatic development, has been shown to render mouse NIH/3T3 cells tumorigenic in SCID mice, have been identified as proteins related to Drosophila fringe, though all are expressed in the mouse embryo as well as in many adult tissues, Three mammalian fringe family members |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Manic Fringe/MFNG Polyclonal Antibody |
| Other name: |
Anti-Manic Fringe/MFNG Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Manic Fringe/MFNG |
| Short name: |
Anti-Manic Fringe/MFNG (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Manic Fringe/MFNG (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7038 |
| Gene: |
MFNG |
More about : MFNG |
| Long gene name: |
MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Synonyms gene name: |
manic fringe (Drosophila) homolog manic fringe homolog (Drosophila) |
| Locus: |
22q13, 1 |
| Discovery year: |
1997-11-07 |
| GenBank acession: |
BC094814 |
| Entrez gene record: |
4242 |
| Pubmed identfication: |
9878264 9187150 |
| RefSeq identity: |
NM_002405 |
| Classification: |
Beta 3-glycosyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000150560 |