| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BRCA2 |
| Specificity: |
This antibody reacts specifically with BRCA2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human BRCA2 |
| Gene ID: |
675 |
| Swiss Prot: |
P51587 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts by targeting RAD51 to ssDNA over double-stranded DNA, Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA), Binds selectively to ssDNA, In concert with NPM1, Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and DSS1, May participate in S phase checkpoint activation, May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks, Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR, Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability, and to ssDNA in tailed duplexes and replication fork structures, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis, independently of its known role in homologous recombination (PubMed:24896180), regulates centrosome duplication, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity, Involved in double-strand break repair and/or homologous recombination |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FAD FAD1 BRCC2 XRCC11 |
| Also known as: |
BRCA2 Polyclonal Antibody |
| Other name: |
Anti-BRCA2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BRCA2 |
| Short name: |
Anti-BRCA2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, early onset (polyclonal), antibody to-breast cancer 2 |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BRCA2 and IDBG-22703 and ENSG00000139618 and 675, BRCA2 and IDBG-630223 and ENSBTAG00000000988 and, BRCC2 and BROVCA2 and FACD and FAD and FAD1 and FANCD and FANCD1 and GLM3 and PNCA2, Brca2 and IDBG-209587 and ENSMUSG00000041147 and 12190, DNA-templated and biological process this GO :0045931 and positive regulation of mitotic cell cycle and biological process this GO :0048478 and replication fork protection and biological process this GO :0051276 and chromosome organization and biological process this GO :0051298 and centrosome duplication and biological process, early onset, gamma-tubulin binding, nuclei, signal transduction by p53 class mediator resulting in transcription of p21 class mediator and biological process this GO :0007141 and male meiosis I and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007420 and brain development and biological process this GO :0007569 and cell aging and biological process this GO :0008283 and cell proliferation and biological process this GO :0008585 and female this GO nad development and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0010165 and response to X-ray and biological process this GO :0010225 and response to UV-C and biological process this GO :0010332 and response to gamma radiation and biological process this GO :0010484 and H3 histone acetyltransferase activity and molecular function this GO :0010485 and H4 histone acetyltransferase activity and molecular function this GO :0030097 and hemopoiesis and biological process this GO :0030141 and secretory granule and cellular component this GO :0032465 and regulation of cytokinesis and biological process this GO :0033593 and BRCA2-MAGE-D1 complex and cellular component this GO :0033600 and negative regulation of mammary gland epithelial cell proliferation and biological process this GO :0042771 and intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and biological process this GO :0043009 and chordate embryonic development and biological process this GO :0043015 and gamma-tubulin binding and molecular function this GO :0043234 and protein complex and cellular component this GO :0043966 and histone H3 acetylation and biological process this GO :0043967 and histone H4 acetylation and biological process this GO :0045893 and positive regulation of transcription, this GO :0000724 and double-strand break repair via homolo this GO us recombination and biological process this GO :0000910 and cytokinesis and biological process this GO :0001556 and oocyte maturation and biological process this GO :0001833 and inner cell mass cell proliferation and biological process this GO :0002020 and protease binding and molecular function this GO :0003697 and single-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005813 and centrosome and cellular component this GO :0006281 and DNA repair and biological process this GO :0006289 and nucleotide-excision repair and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006978 and DNA damage response, this GO :0002020 : protease binding, this GO :0002020 : protease binding and also this GO :0003697 : single-stranded DNA binding and also this GO :0005515 : protein binding and also this GO :0010484 : H3 histone acetyltransferase activity and also this GO :0010485 : H4 histone acetyltransferase activity and also this GO :0043015 : gamma-tubulin binding, this GO :0003697 : single-stranded DNA binding, this GO :0005515 : protein binding, this GO :0010484 : H3 histone acetyltransferase activity, this GO :0010485 : H4 histone acetyltransferase activity, this GO :0043015 : gamma-tubulin binding, breast cancer 2 |
| Identity: |
1101 |
| Gene: |
BRCA2 |
More about : BRCA2 |
| Long gene name: |
BRCA2, DNA repair associated |
| Synonyms gene: |
FANCD1 FACD FANCD |
| Synonyms gene name: |
Fanconi anemia, complementation group D1 breast cancer 2, early onset breast cancer 2 |
| Synonyms name: |
BRCA1/BRCA2-containing complex, subunit 2 |
| Locus: |
13q13, 1 |
| Discovery year: |
1994-10-17 |
| GenBank acession: |
U43746 |
| Entrez gene record: |
675 |
| Pubmed identfication: |
8091231 7581463 15057823 |
| RefSeq identity: |
NM_000059 |
| Classification: |
Fanconi anemia complementation groups |
| Havana BLAST/BLAT: |
OTTHUMG00000017411 |
| Locus Specific Databases: |
Breast Cancer Fanconi Anaemia Mutation Database BRCA1 database at LOVD-China LOVD - Leiden Open Variation Database LOVD - Leiden Open Variation Database Fanconi anemia database LRG_293 |