| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Radical Fringe |
| Specificity: |
This antibody reacts specifically with Radical Fringe |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Radical Fringe |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Each mammalian Fringe displays different patterns of expression, Fringe proteins act upstream of the Notch signaling pathway and are involved in boundary determination during segmentation, Lunatic Fringe is required for normal somite segmentation and patterning and is thought to be a target of the molecular clock, Manic, Manic Fringe, Playing a key role in the development of the limb bud, Radical Fringe, Radical Fringe transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor, Radical and Lunatic Fringe, a protein involved in development, also involved in somatic development, also known as Beta-1, has been shown to render mouse NIH/3T3 cells tumorigenic in SCID mice, have been identified as proteins related to Drosophila Fringe, is a 331 amino acid single-pass type II membrane protein that localizes to the membrane of the Golgi apparatus, though all are expressed in the mouse embryo as well as in many adult tissues, 3-N-acetylglucosaminyltransferase Radical Fringe, Three mammalian fringe family members |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Radical Fringe Polyclonal Antibody |
| Other name: |
Anti-Radical Fringe Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Radical Fringe |
| Short name: |
Anti-Radical Fringe (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Radical Fringe (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9974 |
| Gene: |
RFNG |
More about : RFNG |
| Long gene name: |
RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Synonyms gene name: |
radical fringe (Drosophila) homolog radical fringe homolog (Drosophila) |
| Locus: |
17q25, 3 |
| Discovery year: |
1997-11-07 |
| GenBank acession: |
BC069034 |
| Entrez gene record: |
5986 |
| Pubmed identfication: |
9187150 |
| RefSeq identity: |
NM_002917 |
| Classification: |
Beta 3-glycosyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000178512 |