| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NFIA/NF-1 |
| Specificity: |
This antibody reacts specifically with NFIA/NF-1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NFIA/NF-1 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene, GRP 78, HSP 70, Histone H1, NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes, alpha-globin, also designated CTF, and activates transcription through a putatively novel, carboxy terminal transactivation domain, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription, including that of Ha-Ras, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter, proline-rich, NF-1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NFI-L KIAA1439 |
| Also known as: |
NFIA/NF-1 Polyclonal Antibody |
| Other name: |
Anti-NFIA/NF-1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NFIA/NF-1 |
| Short name: |
Anti-NFIA/NF-1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NFIA/NF-1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7784 |
| Gene: |
NFIA |
More about : NFIA |
| Long gene name: |
nuclear factor I A |
| Synonyms gene name: |
nuclear factor I/A |
| Locus: |
1p31, 3 |
| Discovery year: |
1995-03-09 |
| GenBank acession: |
U07809 |
| Entrez gene record: |
4774 |
| Pubmed identfication: |
7590749 |
| RefSeq identity: |
NM_005595 |
| Havana BLAST/BLAT: |
OTTHUMG00000008618 |