| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RhoA/C |
| Specificity: |
This antibody reacts specifically with RhoA/CPolyclonal |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Helical, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human RhoA |
| Gene ID: |
387, 389 |
| Swiss Prot: |
P61586 |
| Applications: |
IF(ICC), IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activated by ARHGEF2, Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly, In turn, Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, It controls the localization of APC and CLASP2 to the cell membrane, May be an activator of PLCE1, Plays an essential role in cleavage furrow formation, Required for the apical junction formation of keratinocyte cell-cell adhesion, Serves as a target for the yopT cysteine peptidase from Yersinia pestis, Stimulates PKN2 kinase activity, The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex, and Yersinia pseudotuberculosis, membrane-bound APC allows the localization of the MACF1 to the cell membrane, vector of the plague, via the regulation of GSK3B activity, which causes gastrointestinal disorders, which is required for microtubule capture and stabilization, which promotes the exchange of GDP for GTP, Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ARH12, ARH9, ARHC, RHO12, RHOA, RHOH12, Rho cDNA clone 12, Rho cDNA clone 9, Rho-related GTP-binding protein RhoC, Transforming protein RhoA, h12, ARHA |
| Also known as: |
RhoA/CPolyclonal Antibody |
| Other name: |
Anti-RhoA/CPolyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RhoA/C |
| Short name: |
Anti-RhoA/C(Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ras homolog family member A/C(polyclonal) |
| Alternative technique: |
polyclonals |