| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
VGF/NERP1 |
| Specificity: |
This antibody reacts specifically with VGF/NERP1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NERP1 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Its expression is rapidly induced by injury, Nerve growth factor (NGF) is a peptide that plays a key role in the differentiation and survival of neurons in the peripheral nervous system (PNS) and the central nervous system (CNS), The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, The structural organization of this gene is similar to that of the rat gene, VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line, VGF is widely expressed in both the PNS and CNS, VGF plays an essential role in how the brain regulates energy expenditure and body weight, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene, and is upregulated by nerve growth factor, and neuronal activity, but is especially abundant in the adult hypothalamus, however, its exact function is not known, the circadian clock, This gene is specifically expressed in a subpopulation of neuroendocrine cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SCG7 SgVII |
| Also known as: |
VGF/NERP1 Polyclonal Antibody |
| Other name: |
Anti-VGF/NERP1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
VGF/NERP1 |
| Short name: |
Anti-VGF/NERP1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-VGF/NERP1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12684 |
| Gene: |
VGF |
More about : VGF |
| Long gene name: |
VGF nerve growth factor inducible |
| Synonyms name: |
neuro-endocrine specific protein VGF |
| Locus: |
7q22, 1 |
| Discovery year: |
1997-07-22 |
| GenBank acession: |
Y12661 |
| Entrez gene record: |
7425 |
| Pubmed identfication: |
9344675 |
| RefSeq identity: |
NM_003378 |
| Classification: |
Granins |
| Havana BLAST/BLAT: |
OTTHUMG00000157109 |