| Category: |
Antibody |
| Concentration: |
pH7, with 0, 02% sodium azide, 3, 5 mg/ml in 1X TBS, 5% BSA (US sourced) and 0 |
| Form: |
Purified antibody |
| Conjugation: |
Biotinylated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
GAPDH Loading Control [Biotin Conjugate] |
| Host animal: |
Goat (Capra aegagrus hircus) |
| Clonality: |
Polyclonal (goat origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Pig , Rat, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA (peptide), ICC, IHC, WB |
| Recommended dilutions: |
3-1ug/ml, ELISA (peptide) LOD: 1:64000, Immunocytochemistry: suitable, Immunohistochemistry (FFPE): suitable, Western blot: 0 |
| Notes: |
A non-animal protein (NAP) blocker is recommended, Do not use a blocking agent or diluent that contains biotin (including milk and some BSA preparations) when testing with biotinylated antibodies, <, 1, Optimal dilution of the GAPDH loading control antibody should be determined by the researcher, p> |
| Intented use: |
This GAPDH loading control antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
2597 |
| Purity: |
Antigen affinity |
| Description: |
GAPDH, Additional name(s) for this target protein: Glyceraldehyde-3-phosphate dehydrogenase |
| Immunogen: |
Amino acids HQVVSSDFNSDT were used as the immunogen for this GAPDH loading control antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot and store the GAPDH loading control antibody at -20 deg |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
GAPDH Loading [ Conjugate] |
| Short name: |
GAPDH Loading Control Antibody [ Conjugate] |
| Technique: |
antibodies against human proteins, antibodies for, controls, Antibody |
| Label: |
Biotin |
| Alternative name: |
GAPDH Loading reference (Antibody to) [biotinilated Conjugate] |
| Alternative technique: |
antibodies |
| Identity: |
4141 |
| Gene: |
GAPDH |
More about : GAPDH |
| Long gene name: |
glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms gene: |
GAPD |
| Locus: |
12p13, 31 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
AF261085 |
| Entrez gene record: |
2597 |
| Pubmed identfication: |
3170585 |
| RefSeq identity: |
NM_002046 |
| Havana BLAST/BLAT: |
OTTHUMG00000137379 |