| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
TFIID/TATA binding protein TBP |
| Specificity: |
This antibody reacts specifically with TFIID/TATA binding protein TBP |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human TATA binding protein TBP |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binding of TFIID to the TATA element initiates assembly of the other factors into a pre-initiation complex, Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, The TATA-binding subunit of TFIID (designated TFIIDt or TBP) from higher eukaryotes contains a highly conserved 180 amino acid C-terminal domain, The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site, and sequence-specific factors that regulate gene expression, including TFIIA, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors, In eukaryotic systems |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
TFIID |
| Also known as: |
TFIID/TATA binding protein TBP Antibody |
| Other name: |
Anti-TFIID/TATA binding protein TBP |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
TFIID/TATA binding protein TBP |
| Short name: |
Fluor 594, Anti-TFIID/TATA binding protein TBP |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-TFIID/TATA binding protein TBP |
| Identity: |
11588 |
| Gene: |
TBP |
More about : TBP |
| Long gene name: |
TATA-box binding protein |
| Synonyms gene: |
GTF2D1 SCA17 |
| Locus: |
6q27 |
| Discovery year: |
1993-05-26 |
| GenBank acession: |
M55654 |
| Entrez gene record: |
6908 |
| Pubmed identfication: |
2194289 11448935 |
| RefSeq identity: |
NM_003194 |
| Classification: |
General transcription factors |
| Havana BLAST/BLAT: |
OTTHUMG00000016084 |