| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NDUFS7 |
| Specificity: |
This antibody reacts specifically with NDUFS7 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NDUFS7 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
As a subunit of mitochondrial complex I, By oxidizing NADH that is produced in the Krebs cycle, Defects in the gene encoding NDUFS7 are the cause of Leigh syndrome, Mitochondrial complex I consists of over 40 subunits and is of considerable clinical interest since defects in any of the subunits can lead to various myopathies and neuropathies, NDUFS7 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 7), a severe neurological disorder that is characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions, also designated NADH-ubiquinone oxidoreductase 20 kDa subunit, is a 213 amino acid protein that is suggested to be required for catalytic activity, mitochondrial complex I is the first and largest enzyme in the electron transport chain of oxidative phosphorylation, thereby initiating the passage of electrons to successive complexes and ultimately leading to the reduction of oxygen to water, this complex utilizes the two electrons to reduce ubiquinone to ubiquinol, Located in the mitochondrial inner membrane |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PSST FLJ46880 FLJ45860 CI-20 |
| Also known as: |
NDUFS7 Polyclonal Antibody |
| Other name: |
Anti-NDUFS7 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NDUFS7 |
| Short name: |
Anti-NDUFS7 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NDUFS7 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7714 |
| Gene: |
NDUFS7 |
More about : NDUFS7 |
| Long gene name: |
NADH:ubiquinone oxidoreductase core subunit S7 |
| Synonyms gene name: |
20kDa (NADH-coenzyme Q reductase) , NADH dehydrogenase (ubiquinone) Fe-S protein 7 (20kD) (NADH-coenzyme Q reductase) NADH dehydrogenase (ubiquinone) Fe-S protein 7 |
| Synonyms name: |
complex I 20kDa subunit NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial |
| Locus: |
19p13, 3 |
| Discovery year: |
1996-07-26 |
| GenBank acession: |
AF115969 |
| Entrez gene record: |
374291 |
| Pubmed identfication: |
8938450 |
| RefSeq identity: |
NM_024407 |
| Classification: |
NADH:ubiquinone oxidoreductase core subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000168077 |