| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
TPPP |
| Specificity: |
This antibody reacts specifically with TPPP |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human TPPP |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Microtubules are structural components that play important roles in mitosis, TPPP (Tubulin polymerization-promoting protein), TPPP is phosphorylated by TPK II and is encoded by a gene that maps to human chromosome 5, TPPP may form dimers and functions in polymerizing tubulin into double-walled tubules, TPPP overexpression prevents formation of the mitotic spindle assembly and breakdown of the nuclear envelope, also known as p24 and p25, cytokinesis and vesicle transport, is a widely expressed 219 amino acid protein found in the perinuclear region of the cytoplasm, or stabilized blocks, polymorphic aggregates, which contains 181 million base pairs and comprises nearly 6% of the human genome, Tubulin family members are globular proteins important in the assembly of microtubules |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
p25alpha TPPP1 p25 TPPP/p25 |
| Also known as: |
TPPP Polyclonal Antibody |
| Other name: |
Anti-TPPP Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
TPPP |
| Short name: |
Anti-TPPP (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-TPPP (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
24164 |
| Gene: |
TPPP |
More about : TPPP |
| Long gene name: |
tubulin polymerization promoting protein |
| Synonyms name: |
brain specific protein p25 alpha |
| Locus: |
5p15, 33 |
| Discovery year: |
2007-05-02 |
| GenBank acession: |
AB017016 |
| Entrez gene record: |
11076 |
| Pubmed identfication: |
10083737 12093283 15590652 17105200 |
| RefSeq identity: |
NM_007030 |
| Classification: |
Tubulin polymerization promoting proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000131011 |