| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PICALM/CALM |
| Specificity: |
This antibody reacts specifically with PICALM/CALM |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PICALM/CALM |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
AP180 (also designated AP-3 or F1-20) is a synapse-specific clathrin assembly protein, Alpha-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues, The AP-1 and AP-2 adapter protein complexes consist of clathrin binding adaptin proteins and two smaller subunits known as AP50 and AP17, The Alpha- and Beta-Adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length, The protein CALM (clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in clathrin assembly, Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex, Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with clathrin associated protein complexes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CALM CLTH |
| Also known as: |
PICALM/CALM Polyclonal Antibody |
| Other name: |
Anti-PICALM/CALM Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PICALM/CALM |
| Short name: |
Anti-PICALM/CALM (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-phosphatidylinositol binding clathrin assembly protein/CALM (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
CALM and CLTH and LAP, DNA-templated and biological process this GO :0048261 and negative regulation of receptor-mediated endocytosis and biological process this GO :0048268 and clathrin coat assembly and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0048813 and dendrite morphogenesis and biological process this GO :0055072 and iron ion homeostasis and biological process this GO :0072583 and clathrin-mediated endocytosis and biological process this GO :0097418 and neurofibrillary tangle and cellular component this GO :0097459 and iron ion import into cell and biological process this GO :1901216 and positive regulation of neuron death and biological process this GO :1902004 and positive regulation of beta-amyloid formation and biological process this GO :1902959 and regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process and biological process this GO :1902961 and positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process and biological process this GO :1902963 and negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process and biological process, PICALM and IDBG-641926 and ENSBTAG00000001657 and 513579, PICALM and IDBG-66712 and ENSG00000073921 and 8301, Picalm and IDBG-198693 and ENSMUSG00000039361 and 233489, clathrin adaptor activity, nuclei, this GO :0005515 and protein binding and molecular function this GO :0005543 and phospholipid binding and molecular function this GO :0005545 and 1-phosphatidylinositol binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005905 and coated pit and cellular component this GO :0006461 and protein complex assembly and biological process this GO :0006898 and receptor-mediated endocytosis and biological process this GO :0007409 and axonogenesis and biological process this GO :0008283 and cell proliferation and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0016020 and membrane and cellular component this GO :0016188 and synaptic vesicle maturation and biological process this GO :0016192 and vesicle-mediated transport and biological process this GO :0016197 and endosomal transport and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030100 and regulation of endocytosis and biological process this GO :0030118 and clathrin coat and cellular component this GO :0030122 and AP-2 adaptor complex and cellular component this GO :0030132 and clathrin coat of coated pit and cellular component this GO :0030276 and clathrin binding and molecular function this GO :0031623 and receptor internalization and biological process this GO :0031982 and vesicle and cellular component this GO :0032050 and clathrin heavy chain binding and molecular function this GO :0032880 and regulation of protein localization and biological process this GO :0035459 and car this GO loading into vesicle and biological process this GO :0035615 and clathrin adaptor activity and molecular function this GO :0042734 and presynaptic membrane and cellular component this GO :0043025 and neuronal cell body and cellular component this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0045211 and postsynaptic membrane and cellular component this GO :0045893 and positive regulation of transcription, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0005543 : phospholipid binding and also this GO :0005545 : 1-phosphatidylinositol binding and also this GO :0030276 : clathrin binding and also this GO :0032050 : clathrin heavy chain binding and also this GO :0035615 : clathrin adaptor activity, this GO :0005543 : phospholipid binding, this GO :0005545 : 1-phosphatidylinositol binding, this GO :0030276 : clathrin binding, this GO :0032050 : clathrin heavy chain binding, this GO :0035615 : clathrin adaptor activity, phosphatidylinositol binding clathrin assembly protein |
| Identity: |
15514 |
| Gene: |
PICALM |
More about : PICALM |
| Long gene name: |
phosphatidylinositol binding clathrin assembly protein |
| Locus: |
11q14, 2 |
| Discovery year: |
2001-04-06 |
| GenBank acession: |
BC048259 |
| Entrez gene record: |
8301 |
| Pubmed identfication: |
8643484 |
| RefSeq identity: |
NM_007166 |
| Havana BLAST/BLAT: |
OTTHUMG00000166981 |