| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HCN2 + HCN4 |
| Specificity: |
This antibody reacts specifically with HCN2 + HCN4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HCN2 + HCN4 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
HCN currents have been linked to pacemaker activity in the heart and brain, HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells, HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides, HCN2 is highly abundant in mamillary bodies, HCN4 is another member of the family of hyperpolarization activated and cyclic nucleotide gated channels, It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node, and nucleus of the trapezoid body, as well as, as well as neuronal plasticity, contribute to spontaneous rhythmic activity in both heart and brain, pontine nucleus, resting potential control, shaping autonomous activity of single neurons and the periodicity of network oscillations, ventral cochlear nucleus, Hyperpolarization activated cation channels of the HCN gene family such as HCN2 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
HCN2 + HCN4 Polyclonal Antibody |
| Other name: |
Anti-HCN2 + HCN4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HCN2 + HCN4 |
| Short name: |
Anti-HCN2 + HCN4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HCN2 and HCN4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
16882 |
| Gene: |
HCN4 |
More about : HCN4 |
| Long gene name: |
hyperpolarization activated cyclic nucleotide gated potassium channel 4 |
| Synonyms gene name: |
hyperpolarization activated cyclic nucleotide-gated potassium channel 4 |
| Locus: |
15q24, 1 |
| Discovery year: |
2002-09-02 |
| GenBank acession: |
AJ132429 |
| Entrez gene record: |
10021 |
| Pubmed identfication: |
10228147 10430953 16382102 |
| RefSeq identity: |
NM_005477 |
| Classification: |
Cyclic nucleotide gated channels |
| Havana BLAST/BLAT: |
OTTHUMG00000137563 |