| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Gemin 3/SMA |
| Specificity: |
This antibody reacts specifically with Gemin 3/SMA |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Gemin 3 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
DDX20, DEAD-box protein DP130 and DEAD/H box 20, Gemin3, Gemin3 also interacts with SmB, Gemin3 is a putative RNA helicase and shows ATPase activity, Gemin4, Gemin5 and Gemin6, It associates directly with SMN and is a part of the SMN complex containing Gemin2, It contains the conserved motif Asp-Glu-Ala-Asp (DEAD) characteristic of DEAD-box proteins, It is expressed in B and T cell neuroblastoma-derived cell lines, It is found in both the cytoplasm and the nucleus, SMA is caused by deletion or loss-of-function mutations in the SMN (survival of motor neuron) gene, SmD2 and SmD3, The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus, The nuclear form is concentrated in subnuclear bodies called gems (for Gemini of the coiled bodies), also known as DP103, as well as several spliceosomal snRNP proteins, is a protein product of human chromosome 1p13, kidney and lung, liver, malignant melanoma tumor, normal testis and is expressed in low levels in colon, skeletal muscle, 2, Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
BCD541 SMNT SMA1 SMA2 SMA3 GEMIN1 TDRD16A |
| Also known as: |
Gemin 3/SMA Polyclonal Antibody |
| Other name: |
Anti-Gemin 3/SMA Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Gemin 3/SMA |
| Short name: |
Anti-Gemin 3/SMA (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Gemin 3/SMA (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11117 |
| Gene: |
SMN1 |
More about : SMN1 |
| Long gene name: |
survival of motor neuron 1, telomeric |
| Synonyms gene: |
SMA@ SMA |
| Synonyms gene name: |
Kugelberg-Welander disease) , spinal muscular atrophy (Werdnig-Hoffmann disease |
| Synonyms name: |
gemin-1 tudor domain containing 16A |
| Locus: |
5q13, 2 |
| Discovery year: |
1996-12-12 |
| GenBank acession: |
U18423 |
| Entrez gene record: |
6606 |
| Pubmed identfication: |
7813012 |
| RefSeq identity: |
NM_000344 |
| Classification: |
Tudor domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000099361 |
| Locus Specific Databases: |
SMN1 - Spinal Muscular Atrophy (SMA) Leiden Muscular Dystrophy pages ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database LRG_676 |