| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GCNF |
| Specificity: |
This antibody reacts specifically with GCNF |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GCNF |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
GCNF can homodimerize and bind DNA, GCNF is present in spermatocytes and round spermatids of adult male mouse testis, GCNF regulates paracrine interaction between the oocyte and somatic cells by regulating the expression of BMP-15 and GDF-9, The gene expresses three alternatively spliced transcript variants, northern blot and ribonuclease protection assays have shown GCNF is predominant in the testis, to affect female fertility, Germ cell nuclear factor (GCNF) is an orphan member of the nuclear receptor gene superfamily that influences neurogenesis and germ cell development |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
GCNF1 RTR CT150 |
| Also known as: |
GCNF Polyclonal Antibody |
| Other name: |
Anti-GCNF Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GCNF |
| Short name: |
Anti-GCNF (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GCNF (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7985 |
| Gene: |
NR6A1 |
More about : NR6A1 |
| Long gene name: |
nuclear receptor subfamily 6 group A member 1 |
| Synonyms gene: |
GCNF |
| Synonyms gene name: |
group A, member 1 , nuclear receptor subfamily 6 |
| Locus: |
9q33, 3 |
| Discovery year: |
1997-08-22 |
| GenBank acession: |
U64876 |
| Entrez gene record: |
2649 |
| Pubmed identfication: |
9134503 8982251 |
| Classification: |
Nuclear hormone receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000020661 |