| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Slit1/MEGF4 |
| Specificity: |
This antibody reacts specifically with Slit1/MEGF4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Slit1/MEGF4 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
2), Roundabout (Robo) (1, Slit is expressed in the midline of the central nervous system in both vertebrates and invertebrates, Slit proteins are expressed in equivalent regions, Slit2 is a short range inhibitory guidance cue for retinal ganglion cell (RGC) axons that may mediate spatial progression of RGCs (6, Within the developing central nervous system (CNS) of different vertebrate systems, suggesting a conserved function among vertebrate homologs (3, where it functions as a regulatory factor of mesodermal cell movement during gastrulation (5), 4), 7), Secreted leucine-rich repeat-containing proteins 1-3 (Slit1-3) are secreted glycoproteins that influence axonal guidance and mediate normal neural development by acting as high-affinity signaling ligands for the repulsive guidance receptor |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
slit1 MEGF4 Slit-1 SLIT3 |
| Also known as: |
Slit1/MEGF4 Polyclonal Antibody |
| Other name: |
Anti-Slit1/MEGF4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Slit1/MEGF4 |
| Short name: |
Anti-Slit1/MEGF4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-slit homolog 1 (Drosophila)/MEGF4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11085 |
| Gene: |
SLIT1 |
More about : SLIT1 |
| Long gene name: |
slit guidance ligand 1 |
| Synonyms gene: |
SLIL1 |
| Synonyms gene name: |
slit (Drosophila) homolog 1 slit homolog 1 (Drosophila) |
| Locus: |
10q24, 1 |
| Discovery year: |
1998-03-25 |
| GenBank acession: |
AB011537 |
| Entrez gene record: |
6585 |
| Pubmed identfication: |
9693030 9813312 |
| RefSeq identity: |
NM_003061 |
| Havana BLAST/BLAT: |
OTTHUMG00000018843 |