| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ARF6 |
| Specificity: |
This antibody reacts specifically with ARF6 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human ARF6 |
| Gene ID: |
382 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A pseudogene of this gene is located on chromosome 7, The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D, The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, and signaling pathways that lead to actin remodeling, remodelling of membrane lipids, which is part of the RAS superfamily, This gene encodes a member of the human ARF gene family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
ARF6 Polyclonal Antibody |
| Other name: |
Anti-ARF6 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ARF6 |
| Short name: |
Anti-ARF6 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ADP-ribosylation factor 6 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
ARF6 and IDBG-5864 and ENSG00000165527 and 382, ARF6 and IDBG-648584 and ENSBTAG00000045980 and 100294979, Arf6 and IDBG-144539 and ENSMUSG00000044147 and 11845, nuclei, thioesterase binding, this GO :0001726 and ruffle and cellular component this GO :0001889 and liver development and biological process this GO :0003924 and GTPase activity and molecular function this GO :0004871 and signal transducer activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005525 and GTP binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005768 and endosome and cellular component this GO :0005769 and early endosome and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005938 and cell cortex and cellular component this GO :0006184 and GTP catabolic process and biological process this GO :0006886 and intracellular protein transport and biological process this GO :0006928 and cellular component movement and biological process this GO :0007049 and cell cycle and biological process this GO :0007155 and cell adhesion and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0015031 and protein transport and biological process this GO :0016020 and membrane and cellular component this GO :0016192 and vesicle-mediated transport and biological process this GO :0019001 and guanyl nucleotide binding and molecular function this GO :0030139 and endocytic vesicle and cellular component this GO :0030496 and midbody and cellular component this GO :0030838 and positive regulation of actin filament polymerization and biological process this GO :0030866 and cortical actin cytoskeleton organization and biological process this GO :0031527 and filopodium membrane and cellular component this GO :0031529 and ruffle organization and biological process this GO :0031683 and G-protein beta/gamma-subunit complex binding and molecular function this GO :0031996 and thioesterase binding and molecular function this GO :0032154 and cleavage furrow and cellular component this GO :0033028 and myeloid cell apoptotic process and biological process this GO :0034394 and protein localization to cell surface and biological process this GO :0035020 and regulation of Rac protein signal transduction and biological process this GO :0036010 and protein localization to endosome and biological process this GO :0045087 and innate immune response and biological process this GO :0048261 and negative regulation of receptor-mediated endocytosis and biological process this GO :0051301 and cell division and biological process this GO :0051489 and regulation of filopodium assembly and biological process this GO :0055037 and recycling endosome and cellular component this GO :0055038 and recycling endosome membrane and cellular component this GO :0060998 and regulation of dendritic spine development and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0090004 and positive regulation of establishment of protein localization to plasma membrane and biological process this GO :0090162 and establishment of epithelial cell polarity and biological process this GO :0097284 and hepatocyte apoptotic process and biological process, this GO :0003924 : GTPase activity, this GO :0003924 : GTPase activity and also this GO :0004871 : signal transducer activity and also this GO :0005515 : protein binding and also this GO :0005525 : GTP binding and also this GO :0019001 : guanyl nucleotide binding and also this GO :0031683 : G-protein beta/gamma-subunit complex binding and also this GO :0031996 : thioesterase binding, this GO :0004871 : signal transducer activity, this GO :0005515 : protein binding, this GO :0005525 : GTP binding, this GO :0019001 : guanyl nucleotide binding, this GO :0031683 : G-protein beta/gamma-subunit complex binding, this GO :0031996 : thioesterase binding, ADP-ribosylation factor 6 |
| Identity: |
659 |
| Gene: |
ARF6 |
More about : ARF6 |
| Long gene name: |
ADP ribosylation factor 6 |
| Locus: |
14q21, 3 |
| Discovery year: |
1994-02-01 |
| Entrez gene record: |
382 |
| Pubmed identfication: |
1993656 10343114 |
| RefSeq identity: |
NM_001663 |
| Classification: |
ARF GTPase family |
| Havana BLAST/BLAT: |
OTTHUMG00000140296 |