| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
VAMP8/Endobrevin |
| Specificity: |
This antibody reacts specifically with VAMP8 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human VAMP8 |
| Gene ID: |
8673 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Endobrevin, Endobrevin interacts specifically with the SNAPs, NSF (N-ethylmaleimide-sensitive factor), SNAPs (soluble NSF attachment proteins) and Synaptotagmin, Similar in sequence to the synaptobrevins, Syntaxins bind to various proteins involved in exocytosis, also designated VAMP-8 or ED, and slightly expressed in brain, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes, endobrevin is abundantly expressed in kidney, including VAMPs (vesicle-associated membrane proteins), is a 100 amino acid single-pass type IV membrane protein that belongs to the synaptobrevin family, moderately expressed in heart and spleen, most likely through an endobrevin-containing SNARE complex, thymus and liver, Syntaxins were originally thought to be docking proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
EDB |
| Also known as: |
VAMP8 Polyclonal Antibody |
| Other name: |
Anti-VAMP8 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
VAMP8 |
| Short name: |
Anti-VAMP8 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-VAMP8 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12647 |
| Gene: |
VAMP8 |
More about : VAMP8 |
| Long gene name: |
vesicle associated membrane protein 8 |
| Synonyms name: |
endobrevin |
| Locus: |
2p11, 2 |
| Discovery year: |
1998-11-30 |
| GenBank acession: |
AF053233 |
| Entrez gene record: |
8673 |
| Pubmed identfication: |
9878266 9614193 |
| RefSeq identity: |
NM_003761 |
| Classification: |
Vesicle associated membrane proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000130180 |