| Antigen detectable with this ELISA kit: |
Calpain 10 (CAPN10) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
103ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain 10 (CAPN10), The concentration of Calpain 10 (CAPN10) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 10 (CAPN10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Calpain 10 (CAPN10), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Calpain 10 (CAPN10) |
| Short name: |
Calpain 10 (CAPN10) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Calpain 10 (calpain 10) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CANP10 and NIDDM1, CAPN10 and IDBG-642358 and ENSBTAG00000002809 and 789674, CAPN10 and IDBG-84773 and ENSG00000142330 and 11132, Capn10 and IDBG-182339 and ENSMUSG00000026270 and 23830, Plasma membranes, cytoskeletal protein binding, this GO :0000149 and SNARE binding and molecular function this GO :0004198 and calcium-dependent cysteine-type endopeptidase activity and molecular function this GO :0005622 and intracellular and cellular component this GO :0005623 and cell and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006508 and proteolysis and biological process this GO :0006921 and cellular component disassembly involved in execution phase of apoptosis and biological process this GO :0008092 and cytoskeletal protein binding and molecular function this GO :0031532 and actin cytoskeleton reorganization and biological process this GO :0032024 and positive regulation of insulin secretion and biological process this GO :0032388 and positive regulation of intracellular transport and biological process this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0046326 and positive regulation of glucose import and biological process this GO :0097050 and type B pancreatic cell apoptotic process and biological process this GO :2000676 and positive regulation of type B pancreatic cell apoptotic process and biological process, this GO :0000149 : SNARE binding, this GO :0000149 : SNARE binding and also this GO :0004198 : calcium-dependent cysteine-type endopeptidase activity and also this GO :0008092 : cytoskeletal protein binding, this GO :0004198 : calcium-dependent cysteine-type endopeptidase activity, this GO :0008092 : cytoskeletal protein binding, calpain 10 |
| Identity: |
1477 |
| Gene: |
CAPN10 |
More about : CAPN10 |
| Long gene name: |
calpain 10 |
| Locus: |
2q37, 3 |
| Discovery year: |
1998-09-25 |
| GenBank acession: |
AF089088 |
| Entrez gene record: |
11132 |
| Pubmed identfication: |
11017071 11018080 |
| RefSeq identity: |
NM_023083 |
| Classification: |
Calpains |
| Havana BLAST/BLAT: |
OTTHUMG00000133358 |