| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PTPN5/STEP |
| Specificity: |
This antibody reacts specifically with PTPN5/STEP |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PTPN5 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Isolated from mouse brain, STEP depresses both NMDAR single-channel activity and synaptic currents, STEP family members are expressed in the dopaminoceptive neurons of the CNS, STEP61 contains a single tyrosine phosphatase domain, STEP61 dephosphorylates Tyr-420 residue and may thus regulate Fyn activity in PSDs, The STEP protein regulates the N-methyl-d-aspartate receptor (NMDAR) complex, The STEP61 protein associates with the Src family kinase member Fyn when Fyn is phosphorylated at Tyr-420 and not Tyr-431, The human STEP gene maps to chromosome 11p15, The membrane-associated STEP61 isoform localizes in the postsynaptic densities (PSDs) of striatal neurons, Upon association, the STEP20 isoform lacks the conserved tyrosine phosphatase domain, two proline-rich domains and two transmembrane domains, with highest expression in the basal ganglia and related structures, 1, 2-p15, The brain-specific STEP (striatal enriched phosphatase) family of protein tyrosine phosphatases (PTPs) comprises both transmembrane and cytosolic protein members which are the products of alternative splicing |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
STEP PTPSTEP STEP61 |
| Also known as: |
PTPN5/STEP Polyclonal Antibody |
| Other name: |
Anti-PTPN5/STEP Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PTPN5/ |
| Short name: |
Anti-PTPN5/STEP (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PTPN5/STEP (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9657 |
| Gene: |
PTPN5 |
More about : PTPN5 |
| Long gene name: |
non-receptor type 5 , protein tyrosine phosphatase |
| Synonyms gene name: |
non-receptor type 5 (striatum-enriched) , protein tyrosine phosphatase |
| Synonyms name: |
striatal-enriched protein tyrosine phosphatase |
| Locus: |
11p15, 1 |
| Discovery year: |
1992-02-12 |
| GenBank acession: |
BC064807 |
| Entrez gene record: |
84867 |
| Pubmed identfication: |
1714595 |
| RefSeq identity: |
NM_001039970 |
| Classification: |
Protein tyrosine phosphatases, non-receptor type |
| Havana BLAST/BLAT: |
OTTHUMG00000134304 |