| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Lamin B2 |
| Specificity: |
This antibody reacts specifically with Lamin B2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Lamin B2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
B-type lamins undergo a series of modifications, Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations, Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family, Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, This family, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity, structure and substrate specificity from any previously described protease family, such as farnesylation and phosphorylation, termed CED-3/ICE, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin, A unique family of Cysteine proteases has been described that differs in sequence |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Lamin B2 Polyclonal Antibody |
| Other name: |
Anti-Lamin B2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Lamin B2 |
| Short name: |
Anti-Lamin B2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Lamin B2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6638 |
| Gene: |
LMNB2 |
More about : LMNB2 |
| Long gene name: |
lamin B2 |
| Synonyms gene: |
LMN2 |
| Locus: |
19p13, 3 |
| Discovery year: |
1992-04-09 |
| GenBank acession: |
M94362 |
| Entrez gene record: |
84823 |
| Pubmed identfication: |
1630457 |
| RefSeq identity: |
NM_032737 |
| Classification: |
Lamins |
| Havana BLAST/BLAT: |
OTTHUMG00000150626 |