| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DAB2 Ser24 |
| Specificity: |
This antibody reacts specifically with DAB2 (Ser24) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser24 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human DAB2 around the phosphorylation site of Ser24 [AP(p-S)KK] |
| Gene ID: |
1601 |
| Swiss Prot: |
P98082 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Can bind and assemble clathrin, Can function in clathrin-mediated endocytosis independently of the AP-2 complex, EPS15L1 and ITSN1, Involved in CFTR trafficking to the late endosome, Involved in TGF-beta receptor signaling and facilitates phosphorylation of the signal transducer SMAD2, Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR, Involved in endocytosis of integrin beta-1, Involved in endocytosis of megalin/LRP2 lipoprotein receptor during embryonal development, Involved in several receptor-mediated signaling pathways, May activate non-canonical Wnt signaling, May inhibit the canoniocal Wnt/beta-catenin signaling pathway by stabilizing the beta-catenin destruction complex through a competing association with axin preventing its dephosphorylation through protein phosphatase 1 (PP1), Mediates TFG-beta-stimulated JNK activation, Required for recycling of the TGF-beta receptor, Sequesters LRP6 towards clathrin-mediated endocytosis, and binds simultaneously to phosphatidylinositol 4, leading to inhibition of Wnt/beta-catenin signaling, such as the LDL receptor, this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, to recruit them to clathrin-coated pits, 5)P2) and cargos containg non-phosphorylated NPXY internalization motifs, 5-bisphosphate (PtdIns(4, Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
DOC-2 |
| Also known as: |
DAB2 (Ser24) Polyclonal Antibody |
| Other name: |
Anti-DAB2 (Ser24) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DAB2 (Ser24) |
| Short name: |
Anti-DAB2 (Ser24) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-DAB2 (Ser24) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
2662 |
| Gene: |
DAB2 |
More about : DAB2 |
| Long gene name: |
DAB2, clathrin adaptor protein |
| Synonyms gene name: |
disabled (Drosophila) homolog 2 (mitogen-responsive phosphoprotein) disabled homolog 2, homolog 2 (Drosophila) , mitogen-responsive phosphoprotein, mitogen-responsive phosphoprotein (Drosophila) Dab |
| Locus: |
5p13, 1 |
| Discovery year: |
1998-07-29 |
| GenBank acession: |
U53446 |
| Entrez gene record: |
1601 |
| Pubmed identfication: |
8660969 9620555 |
| RefSeq identity: |
NM_001343 |
| Havana BLAST/BLAT: |
OTTHUMG00000162043 |