| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Bestrophin 2 |
| Specificity: |
This antibody reacts specifically with Bestrophin 2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Bestrophin 2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
1 also known as Best disease, 3 and 4, Bestrophin 2, Bestrophins were originally defined as a family of over 20 related sequences of the C, Functionally the bestrophines oligomerise to form tetramers and pentamers in order to act as calcium sensitive chloride channels, It has been shown that Bestrophin interacts with beta catalytic subunit of protein phosphatase 2A (PP2Ac), PR65, RT PCR analyses revealed tissue restricted expression of the three genes with both Bestrophin 1 and Bestrophin 2 are abundantly transcribed in colon, Such protein protein interaction between Bestrophin and PP2Ac and the structural subunit of PP2A, The first mammalian Bestrophin was identified as the vitelliform macular dystrophy (VMD), The interaction between PP2Ac and the Bestrophin takes place near the carboxy terminal end of the protein, Three more members of the bestrophin family members were cloned and indentified recently, elegans, was confirmed by reciprocal immunoprecipitation, The Bestrophins are a newly described family of anion channels unrelated in primary sequence to any previously characterized channel proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Bestrophin 2 Polyclonal Antibody |
| Other name: |
Anti-Bestrophin 2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Bestrophin 2 |
| Short name: |
Anti-Bestrophin 2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Bestrophin 2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
17106 |
| Gene: |
BEST4 |
More about : BEST4 |
| Long gene name: |
bestrophin 4 |
| Synonyms gene: |
VMD2L2 |
| Synonyms gene name: |
vitelliform macular dystrophy 2-like 2 |
| Locus: |
1p34, 1 |
| Discovery year: |
2003-12-03 |
| GenBank acession: |
AF440757 |
| Entrez gene record: |
266675 |
| Pubmed identfication: |
12032738 16702355 |
| RefSeq identity: |
NM_153274 |
| Classification: |
Bestrophins |
| Havana BLAST/BLAT: |
OTTHUMG00000008488 |