| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MDM2 |
| Specificity: |
This antibody reacts specifically with MDM2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MDM2 |
| Gene ID: |
4193 |
| Swiss Prot: |
Q00987 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also acts as a ubiquitin ligase E3 toward itself and ARRB1, Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways, Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53, Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation, Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain, Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus, Permits the nuclear export of p53/TP53, Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein, Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation, leading to its degradation by the proteasome, E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HDM2 MGC5370 |
| Also known as: |
MDM2 Polyclonal Antibody |
| Other name: |
Anti-MDM2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MDM2 |
| Short name: |
Anti-MDM2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MDM2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6973 |
| Gene: |
MDM2 |
More about : MDM2 |
| Long gene name: |
MDM2 proto-oncogene |
| Synonyms gene name: |
E3 ubiquitin protein ligase , human homolog of, mouse double minute 2, p53 binding protein (mouse) Mdm2 p53 binding protein homolog (mouse) MDM2 proto-oncogene, p53-binding protein Mdm2, transformed 3T3 cell double minute 2 |
| Locus: |
12q15 |
| Discovery year: |
1993-12-10 |
| Entrez gene record: |
4193 |
| Pubmed identfication: |
1614537 16905769 |
| RefSeq identity: |
NM_006880 |
| Classification: |
Zinc fingers RANBP2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000142827 |