| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
E2F3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Optimal dilution of the E2F3 antibody should be determined by the researcher |
| Added buffer: |
025% sodium azide, 5% BSA and 0, Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2 |
| Intented use: |
This antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O00716 |
| Purity: |
Antigen affinity |
| Description: |
By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22, The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle, The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions, The protein encoded by this gene is a member of the E2F family of transcription factors, This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene, 3, Transcription factor E2F3 |
| Immunogen: |
Amino acids 278-320 (QSCTLDLKLLTEDSENQRLAYVTYQDIRKISGLKDQTVIVVKA-human) were used as the immunogen for the E2F3 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the E2F3 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
E2F3 |
| Short name: |
E2F3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
E2F3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3115 |
| Gene: |
E2F3 |
More about : E2F3 |
| Long gene name: |
E2F transcription factor 3 |
| Locus: |
6p22, 3 |
| Discovery year: |
1994-08-31 |
| GenBank acession: |
Y10479 |
| Entrez gene record: |
1871 |
| Pubmed identfication: |
8246996 |
| Classification: |
E2F transcription factors |
| Havana BLAST/BLAT: |
OTTHUMG00000016389 |