| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Calpastatin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Western blot: 0 |
| Notes: |
Optimal dilution of the Calpastatin antibody should be determined by the researcher |
| Added buffer: |
025% sodium azide, 5% BSA and 0, Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2 |
| Intented use: |
This antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P20810 |
| Purity: |
Antigen affinity |
| Description: |
Alternatively spliced transcript variants encoding different isoforms have been described, It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), It is mapped to 5q15, The calpain/calpastatin system is involved in numerous membrane fusion events, The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins, The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor, and it is involved in the proteolysis of amyloid precursor protein, such as neural vesicle exocytosis and platelet and red-cell aggregation, Calpastatin is a protein that in humans is encoded by the CAST gene |
| Immunogen: |
Amino acids 275-310 (QEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLR-human) were used as the immunogen for the Calpastatin antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Calpastatin antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Calpastatin |
| Short name: |
Calpastatin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Calpastatin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1515 |
| Gene: |
CAST |
More about : CAST |
| Long gene name: |
calpastatin |
| Locus: |
5q15 |
| Discovery year: |
1990-07-03 |
| GenBank acession: |
AF327443 |
| Entrez gene record: |
831 |
| Pubmed identfication: |
8340353 14685690 15820218 |
| RefSeq identity: |
NM_173062 |
| Havana BLAST/BLAT: |
OTTHUMG00000128413 |