| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
WRN / Werner syndrome ATP-dependent helicase |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Added buffer: |
025% sodium azide, 5% BSA and 0, Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2 |
| Intented use: |
This WRN antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q14191 |
| Purity: |
Antigen affinity |
| Description: |
Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, DNA helicases are involved in many aspects of DNA metabolism, Defects in this gene are the cause of Werner syndrome, It possesses an intrinsic 3' to 5' DNA helicase activity, RecQ-like type 3, This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases, This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization, also known as DNA helicase, an autosomal recessive disorder characterized by premature aging, and is also a 3' to 5' exonuclease, and repair, including transcription, is an enzyme that in humans is encoded by the WRN gene, recombination, replication, this protein may be involved in the repair of double strand DNA breaks, Werner syndrome ATP-dependent helicase |
| Immunogen: |
Amino acids Q122-N240 from the human protein were used as the immunogen for the WRN antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the WRN antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
WRN / Werner syndrome ATP-dependent helicase |
| Short name: |
WRN Antibody / Werner syndrome ATP-dependent helicase |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
RecQ helicase-like (Antibody to) / Werner syndrome adenosine triphosphate-dependent helicase, Werner syndrome |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
G-quadruplex DNA binding, RECQ3 and RECQL2 and RECQL3, RecQ helicase-like, WRN and IDBG-16092 and ENSG00000165392 and 7486, WRN and IDBG-628918 and ENSBTAG00000021592 and 505061, Wrn and IDBG-147033 and ENSMUSG00000031583 and 22427, nuclei, this GO :0000166 and nucleotide binding and molecular function this GO :0000287 and magnesium ion binding and molecular function this GO :0000403 and Y-form DNA binding and molecular function this GO :0000405 and bubble DNA binding and molecular function this GO :0000723 and telomere maintenance and biological process this GO :0000731 and DNA synthesis involved in DNA repair and biological process this GO :0001302 and replicative cell aging and biological process this GO :0003676 and nucleic acid binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003678 and DNA helicase activity and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0004003 and ATP-dependent DNA helicase activity and molecular function this GO :0004386 and helicase activity and molecular function this GO :0004527 and exonuclease activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005813 and centrosome and cellular component this GO :0006139 and nucleobase-containing compound metabolic process and biological process this GO :0006200 and ATP catabolic process and biological process this GO :0006259 and DNA metabolic process and biological process this GO :0006260 and DNA replication and biological process this GO :0006281 and DNA repair and biological process this GO :0006284 and base-excision repair and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006310 and DNA recombination and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0007568 and aging and biological process this GO :0007569 and cell aging and biological process this GO :0008026 and ATP-dependent helicase activity and molecular function this GO :0008408 and 3'-5' exonuclease activity and molecular function this GO :0009267 and cellular response to starvation and biological process this GO :0009378 and four-way junction helicase activity and molecular function this GO :0010225 and response to UV-C and biological process this GO :0010259 and multicellular organismal aging and biological process this GO :0016887 and ATPase activity and molecular function this GO :0030145 and manganese ion binding and molecular function this GO :0031297 and replication fork processing and biological process this GO :0032066 and nucleolus to nucleoplasm transport and biological process this GO :0032389 and MutLalpha complex and cellular component this GO :0032403 and protein complex binding and molecular function this GO :0032508 and DNA duplex unwinding and biological process this GO :0040009 and regulation of growth rate and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043138 and 3'-5' DNA helicase activity and molecular function this GO :0043140 and ATP-dependent 3'-5' DNA helicase activity and molecular function this GO :0044237 and cellular metabolic process and biological process this GO :0051345 and positive regulation of hydrolase activity and biological process this GO :0051880 and G-quadruplex DNA binding and molecular function this GO :0071480 and cellular response to gamma radiation and biological process this GO :0090305 and nucleic acid phosphodiester bond hydrolysis and biological process, this GO :0000166 : nucleotide binding, this GO :0000166 : nucleotide binding and also this GO :0000287 : magnesium ion binding and also this GO :0000403 : Y-form DNA binding and also this GO :0000405 : bubble DNA binding and also this GO :0003676 : nucleic acid binding and also this GO :0003677 : DNA binding and also this GO :0003678 : DNA helicase activity and also this GO :0003824 : catalytic activity and also this GO :0004003 : ATP-dependent DNA helicase activity and also this GO :0004386 : helicase activity and also this GO :0004527 : exonuclease activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008026 : ATP-dependent helicase activity and also this GO :0008408 : 3'-5' exonuclease activity and also this GO :0009378 : four-way junction helicase activity and also this GO :0016887 : ATPase activity and also this GO :0030145 : manganese ion binding and also this GO :0032403 : protein complex binding and also this GO :0042803 : protein homodimerization activity and also this GO :0043138 : 3'-5' DNA helicase activity and also this GO :0043140 : ATP-dependent 3'-5' DNA helicase activity and also this GO :0051880 : G-quadruplex DNA binding, this GO :0000287 : magnesium ion binding, this GO :0000403 : Y-form DNA binding, this GO :0000405 : bubble DNA binding, this GO :0003676 : nucleic acid binding, this GO :0003677 : DNA binding, this GO :0003678 : DNA helicase activity, this GO :0003824 : catalytic activity, this GO :0004003 : ATP-dependent DNA helicase activity, this GO :0004386 : helicase activity, this GO :0004527 : exonuclease activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008026 : ATP-dependent helicase activity, this GO :0008408 : 3'-5' exonuclease activity, this GO :0009378 : four-way junction helicase activity, this GO :0016887 : ATPase activity, this GO :0030145 : manganese ion binding, this GO :0032403 : protein complex binding, this GO :0042803 : protein homodimerization activity, this GO :0043138 : 3'-5' DNA helicase activity, this GO :0043140 : ATP-dependent 3'-5' DNA helicase activity, this GO :0051880 : G-quadruplex DNA binding, Werner syndrome |
| Identity: |
12791 |
| Gene: |
WRN |
More about : WRN |
| Long gene name: |
Werner syndrome RecQ like helicase |
| Synonyms gene name: |
Werner syndrome |
| Synonyms: |
RECQL2 RECQ3 |
| Locus: |
8p12 |
| Discovery year: |
1991-08-21 |
| Entrez gene record: |
7486 |
| Pubmed identfication: |
9288107 |
| Classification: |
Exonucleases RecQ like helicases |
| Havana BLAST/BLAT: |
OTTHUMG00000163894 |
| Locus Specific Databases: |
Database of WS-associated WRN mutations LRG_524 |