| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HDAC3/HD3 |
| Specificity: |
This antibody reacts specifically with HDAC3/HD3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HDAC3/HD3 |
| Gene ID: |
8841 |
| Swiss Prot: |
O15379 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation, H2B, H3 and H4), Histone deacetylases act via the formation of large multiprotein complexes, Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1, Required to repress transcription of the POU1F1 transcription factor, and some other non-histone substrates, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression, cell cycle progression and developmental events, increases YY1 repression activity, Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RPD3 HD3 RPD3-2 |
| Also known as: |
HDAC3/HD3 Polyclonal Antibody |
| Other name: |
Anti-HDAC3/HD3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HDAC3/HD3 |
| Short name: |
Anti-HDAC3/HD3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-histone deacetylase 3/HD3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006476 and protein deacetylation and biological process this GO :0007219 and Notch signaling pathway and biological process this GO :0007346 and regulation of mitotic cell cycle and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008152 and metabolic process and biological process this GO :0016568 and chromatin modification and biological process this GO :0016575 and histone deacetylation and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0017053 and transcriptional repressor complex and cellular component this GO :0019899 and enzyme binding and molecular function this GO :0030332 and cyclin binding and molecular function this GO :0031490 and chromatin DNA binding and molecular function this GO :0032041 and NAD-dependent histone deacetylase activity (H3-K14 specific) and molecular function this GO :0032922 and circadian regulation of gene expression and biological process this GO :0033558 and protein deacetylase activity and molecular function this GO :0040014 and regulation of multicellular organism growth and biological process this GO :0042826 and histone deacetylase binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044255 and cellular lipid metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045786 and negative regulation of cell cycle and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0046329 and negative regulation of JNK cascade and biological process this GO :0046969 and NAD-dependent histone deacetylase activity (H3-K9 specific) and molecular function this GO :0046970 and NAD-dependent histone deacetylase activity (H4-K16 specific) and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0051225 and spindle assembly and biological process this GO :0070932 and histone H3 deacetylation and biological process this GO :0070933 and histone H4 deacetylation and biological process this GO :0097372 and NAD-dependent histone deacetylase activity (H3-K18 specific) and molecular function, HD3 and RPD3 and RPD3-2, HDAC3 and IDBG-50725 and ENSG00000171720 and 8841, HDAC3 and IDBG-646121 and ENSBTAG00000017360 and 404125, Hdac3 and IDBG-140814 and ENSMUSG00000024454 and 15183, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, this GO :0000118 and histone deacetylase complex and cellular component this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003714 and transcription corepressor activity and molecular function this GO :0004407 and histone deacetylase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005876 and spindle microtubule and cellular component this GO :0006351 and transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003714 : transcription corepressor activity and also this GO :0004407 : histone deacetylase activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0016787 : hydrolase activity and also this GO :0019899 : enzyme binding and also this GO :0030332 : cyclin binding and also this GO :0031490 : chromatin DNA binding and also this GO :0032041 : NAD-dependent histone deacetylase activity (H3-K14 specific) and also this GO :0033558 : protein deacetylase activity and also this GO :0042826 : histone deacetylase binding and also this GO :0046969 : NAD-dependent histone deacetylase activity (H3-K9 specific) and also this GO :0046970 : NAD-dependent histone deacetylase activity (H4-K16 specific) and also this GO :0097372 : NAD-dependent histone deacetylase activity (H3-K18 specific), this GO :0003682 : chromatin binding, this GO :0003714 : transcription corepressor activity, this GO :0004407 : histone deacetylase activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0016787 : hydrolase activity, this GO :0019899 : enzyme binding, this GO :0030332 : cyclin binding, this GO :0031490 : chromatin DNA binding, this GO :0032041 : NAD-dependent histone deacetylase activity (H3-K14 specific), this GO :0033558 : protein deacetylase activity, this GO :0042826 : histone deacetylase binding, this GO :0046969 : NAD-dependent histone deacetylase activity (H3-K9 specific), this GO :0046970 : NAD-dependent histone deacetylase activity (H4-K16 specific), this GO :0097372 : NAD-dependent histone deacetylase activity (H3-K18 specific), histone deacetylase 3 |
| Identity: |
4854 |
| Gene: |
HDAC3 |
More about : HDAC3 |
| Long gene name: |
histone deacetylase 3 |
| Locus: |
5q31, 3 |
| Discovery year: |
1999-01-29 |
| GenBank acession: |
AF059650 |
| Entrez gene record: |
8841 |
| Pubmed identfication: |
9501169 9464271 |
| RefSeq identity: |
NM_003883 |
| Classification: |
Histone deacetylases, class I |
| Havana BLAST/BLAT: |
OTTHUMG00000129629 |