| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
APOB |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-2ug/ml, IHC (FFPE): 1-2ug/ml, Western blot: 0 |
| Added buffer: |
025% sodium azide, 5% BSA and 0, Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2 |
| Intented use: |
This APOB antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P04114 |
| Purity: |
Antigen affinity |
| Description: |
It occurs in plasma as two main isoforms, Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->, The two isoforms share a common N-terminal sequence, This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins, and early translation termination, and hypercholesterolemia due to ligand-defective apoB, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver, diseases affecting plasma cholesterol and apoB levels, normotriglyceridemic hypobetalipoproteinemia, resulting in the creation of a stop codon, very long mRNA, Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene, UAA) |
| Immunogen: |
This sequence is common to both the full length (ApoB-100) and truncated (ApoB-48) forms of the protein, Amino acids Q246-N450 from the human protein were used as the immunogen for the APOB antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the APOB antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
membranous, Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
APOB |
| Short name: |
APOB Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
apolipoprotein B (including Ag(x) antigen) (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
APOB and IDBG-32746 and ENSG00000084674 and 338, APOB and IDBG-637535 and ENSBTAG00000008505 and 494004, Apob and IDBG-128209 and ENSMUSG00000020609 and 238055, Extracellular, FLDB and LDLCQ4, low-density lipoprotein particle receptor binding, this GO :0001523 and retinoid metabolic process and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0005319 and lipid transporter activity and molecular function this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005543 and phospholipid binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005769 and early endosome and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006629 and lipid metabolic process and biological process this GO :0006642 and triglyceride mobilization and biological process this GO :0006869 and lipid transport and biological process this GO :0006898 and receptor-mediated endocytosis and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007399 and nervous system development and biological process this GO :0007596 and blood coagulation and biological process this GO :0007603 and phototransduction, this GO :0005319 : lipid transporter activity, this GO :0005319 : lipid transporter activity and also this GO :0005488 : binding and also this GO :0005515 : protein binding and also this GO :0005543 : phospholipid binding and also this GO :0008201 : heparin binding and also this GO :0008289 : lipid binding and also this GO :0017127 : cholesterol transporter activity and also this GO :0035473 : lipase binding and also this GO :0050750 : low-density lipoprotein particle receptor binding, this GO :0005488 : binding, this GO :0005515 : protein binding, this GO :0005543 : phospholipid binding, this GO :0008201 : heparin binding, this GO :0008289 : lipid binding, this GO :0017127 : cholesterol transporter activity, this GO :0035473 : lipase binding, this GO :0050750 : low-density lipoprotein particle receptor binding, visible light and biological process this GO :0008201 and heparin binding and molecular function this GO :0008203 and cholesterol metabolic process and biological process this GO :0008289 and lipid binding and molecular function this GO :0009566 and fertilization and biological process this GO :0009615 and response to virus and biological process this GO :0009743 and response to carbohydrate and biological process this GO :0009791 and post-embryonic development and biological process this GO :0010008 and endosome membrane and cellular component this GO :0010033 and response to organic substance and biological process this GO :0010269 and response to selenium ion and biological process this GO :0010744 and positive regulation of macrophage derived foam cell differentiation and biological process this GO :0010884 and positive regulation of lipid storage and biological process this GO :0010886 and positive regulation of cholesterol storage and biological process this GO :0012506 and vesicle membrane and cellular component this GO :0015629 and actin cytoskeleton and cellular component this GO :0016042 and lipid catabolic process and biological process this GO :0017127 and cholesterol transporter activity and molecular function this GO :0019433 and triglyceride catabolic process and biological process this GO :0030301 and cholesterol transport and biological process this GO :0030317 and sperm motility and biological process this GO :0030669 and clathrin-coated endocytic vesicle membrane and cellular component this GO :0031904 and endosome lumen and cellular component this GO :0031983 and vesicle lumen and cellular component this GO :0032496 and response to lipopolysaccharide and biological process this GO :0033344 and cholesterol efflux and biological process this GO :0034359 and mature chylomicron and cellular component this GO :0034360 and chylomicron remnant and cellular component this GO :0034361 and very-low-density lipoprotein particle and cellular component this GO :0034362 and low-density lipoprotein particle and cellular component this GO :0034363 and intermediate-density lipoprotein particle and cellular component this GO :0034374 and low-density lipoprotein particle remodeling and biological process this GO :0034379 and very-low-density lipoprotein particle assembly and biological process this GO :0034383 and low-density lipoprotein particle clearance and biological process this GO :0035473 and lipase binding and molecular function this GO :0042157 and lipoprotein metabolic process and biological process this GO :0042158 and lipoprotein biosynthetic process and biological process this GO :0042159 and lipoprotein catabolic process and biological process this GO :0042627 and chylomicron and cellular component this GO :0042632 and cholesterol homeostasis and biological process this GO :0042953 and lipoprotein transport and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0044281 and small molecule metabolic process and biological process this GO :0045540 and regulation of cholesterol biosynthetic process and biological process this GO :0048844 and artery morphogenesis and biological process this GO :0050750 and low-density lipoprotein particle receptor binding and molecular function this GO :0050900 and leukocyte migration and biological process this GO :0071356 and cellular response to tumor necrosis factor and biological process this GO :0071379 and cellular response to prostaglandin stimulus and biological process this GO :0071682 and endocytic vesicle lumen and cellular component, apolipoprotein B (including Ag(x) antigen) |
| Identity: |
603 |
| Gene: |
APOB |
More about : APOB |
| Long gene name: |
apolipoprotein B |
| Synonyms gene name: |
apolipoprotein B (including Ag(x) antigen) |
| Locus: |
2p24, 1 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M14162 |
| Entrez gene record: |
338 |
| Classification: |
Apolipoproteins |
| Havana BLAST/BLAT: |
OTTHUMG00000090785 |