| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MrpL28 |
| Specificity: |
This antibody reacts specifically with MrpL28 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MrpL28 |
| Gene ID: |
10573 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Among different species, Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA, MRPL28 is a 39S subunit protein, Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit, They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA A24 restricted fashion, and sometimes in biochemical properties, the proteins comprising the mitoribosome differ greatly in sequence, where this ratio is reversed, which prevents easy recognition by sequence homology, Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
p15 |
| Also known as: |
MrpL28 Polyclonal Antibody |
| Other name: |
Anti-MrpL28 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MrpL28 |
| Short name: |
Anti-MrpL28 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MrpL28 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
14484 |
| Gene: |
MRPL28 |
More about : MRPL28 |
| Long gene name: |
mitochondrial ribosomal protein L28 |
| Synonyms gene: |
MAAT1 |
| Synonyms gene name: |
melanoma-associated antigen recognised by cytotoxic T lymphocytes |
| Locus: |
16p13, 3 |
| Discovery year: |
2001-01-26 |
| GenBank acession: |
U19796 |
| Entrez gene record: |
10573 |
| Pubmed identfication: |
11551941 19753307 |
| Classification: |
Mitochondrial ribosomal proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000047994 |