| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PIWIL1 |
| Specificity: |
This antibody reacts specifically with PIWIL1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from chicken PIWIL1 |
| Gene ID: |
416804 |
| Swiss Prot: |
A6N7Y9 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts via the piRNA metabolic process, Besides their function in transposable elements repression, Directly binds methylated piRNAs, a class of 24 to 3 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity), which is essential for the germline integrity, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons, Plays a central role during gametogenesis by repressing transposable elements and preventing their mobilization |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PIWI HIWI CT80, 1 |
| Also known as: |
PIWIL1 Polyclonal Antibody |
| Other name: |
Anti-PIWIL1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PIWIL1 |
| Short name: |
Anti-PIWIL1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PIWIL1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9007 |
| Gene: |
PIWIL1 |
More about : PIWIL1 |
| Long gene name: |
piwi like RNA-mediated gene silencing 1 |
| Synonyms gene name: |
piwi (Drosophila)-like 1 piwi-like 1 (Drosophila) |
| Locus: |
12q24, 33 |
| Discovery year: |
2000-02-11 |
| GenBank acession: |
AF104260 |
| Entrez gene record: |
9271 |
| Pubmed identfication: |
9851978 12906857 |
| Classification: |
Argonaute/PIWI family |
| Havana BLAST/BLAT: |
OTTHUMG00000168382 |