| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
C5a anaphylatoxin |
| Specificity: |
This antibody reacts specifically with C5a anaphylatoxin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Secreted |
| Antigen Source: |
KLH conjugated synthetic peptide derived from mouse C5a (725-770aa) |
| Gene ID: |
15139 |
| Swiss Prot: |
P06684 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, C5 anaphylatoxin is a mediator of local inflammatory process, C5-C9, C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation, C5b has a transient binding site for C6, Derived from proteolytic degradation of complement C5, The C5b-C6 complex is the foundation upon which the lytic complex is assembled, and histamine release from mast cells and basophilic leukocytes, contraction of smooth muscle, increased vascular permeability, into the membrane attack complex, Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CPAMD4 C5a C5b |
| Also known as: |
C5a anaphylatoxin Polyclonal Antibody |
| Other name: |
Anti-C5a anaphylatoxin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
C5a anaphylatoxin |
| Short name: |
Anti-C5a anaphylatoxin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-C5a anaphylatoxin (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1331 |
| Gene: |
C5 |
More about : C5 |
| Long gene name: |
complement C5 |
| Synonyms gene name: |
complement component 5 |
| Synonyms name: |
prepro-C5 C5a anaphylatoxin |
| Locus: |
9q33, 2 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M57729 |
| Entrez gene record: |
727 |
| RefSeq identity: |
NM_001735 |
| Classification: |
Endogenous ligands Complement system C3 and PZP like, alpha-2-macroglobulin domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000020579 |
| Locus Specific Databases: |
C5base: Mutation registry for C5 deficiency LRG_28 |