| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Actin / ACTA1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
1-0, 5-1ug/ml, 5ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Optimal dilution of the Actin antibody should be determined by the researcher |
| Intented use: |
This Actin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P68133 |
| Purity: |
Antigen affinity |
| Description: |
It varies in amount, The actins exhibit over 90% sequence homology, The isoforms are comprised of three alpha-actin, a highly conserved protein, being related to the type of differentiation and to the functional state of cells and tissues, but each isoform has a unique NH2-terminal sequence, is a major component of both the cytoskeletal and contractile structures in the cell types, one beta-actin, two gamma-actin, Actin |
| Immunogen: |
Amino acids HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY of human Actin were used as the immunogen for the Actin antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Actin antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Actin / ACTA1 |
| Short name: |
Actin Antibody / ACTA1 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Actin (Antibody to) / ACTA1 |
| Alternative technique: |
antibodies |
| Identity: |
129 |
| Gene: |
ACTA1 |
More about : ACTA1 |
| Long gene name: |
actin, alpha 1, skeletal muscle |
| Synonyms gene: |
ACTA |
| Synonyms: |
NEM3 |
| Synonyms name: |
nemaline myopathy type 3 |
| Locus: |
1q42, 13 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
J00068 |
| Entrez gene record: |
58 |
| Pubmed identfication: |
10072583 6865942 |
| RefSeq identity: |
NM_001100 |
| Classification: |
Actins |
| Havana BLAST/BLAT: |
OTTHUMG00000038006 |
| Locus Specific Databases: |
Leiden Muscular Dystrophy pages LRG_429 |