| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Mouse (Mus musculus) |
| Target Protein/Peptide: |
KLH |
| Specificity: |
This antibody reacts specifically with KLH |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH protein |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Others |
| Cross Reactive Species details: |
Keyhole limpet hemocyanin (KLH) |
| Background information: |
Aggregates remain immunogenic, Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2), Haptens are substances with a low molecular weight such as peptides, In addition, It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata, Its large size and numerous epitopes generate a substantial immune response, KLH is an effective carrier protein for several reasons, The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein, The large and highly glycosylated KLH protein cannot be reproduced synthetically, When oxygen is bound to hemocyanin, a gastropod, allows a high hapten:carrier protein ratio increasing the likelihood of generating hapten-specific , and abundance of lysine residues for coupling haptens, because KLH is derived from the limpet, biotechnology and therapeutic applications, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350, it is phylogenetically distant from mammalian proteins, opalescent blue color, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production, the molecule takes on a distinctive transparent, thus reducing false positives in immunologically based research techniques in mammalian model organisms, 000, 000, 000 and 390, 000 in aggregates with molecular weights of 4, 000-13, 500, KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate, Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of for research, Keyhole limpet hemocyanin is an extremely large, [2] KLH is the most widely employed carrier proteins for this purpose |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Keyhole limpet hemocyanin KLH |
| Also known as: |
KLH Polyclonal Antibody |
| Other name: |
Anti-KLH Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
KLH |
| Short name: |
Anti-KLH (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-KLH (polyclonal) |
| Alternative technique: |
polyclonals |