| Antigen detectable with this ELISA kit: |
Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2), ATPase |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
126ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Ca++ Transporting, Ca++ Transporting, Ca++ Transporting, Ca++ Transporting, Cardiac Muscle, Cardiac Muscle, Cardiac Muscle, Cardiac Muscle, Next, Slow Twitch 2 (ATP2A2), Slow Twitch 2 (ATP2A2), Slow Twitch 2 (ATP2A2), Slow Twitch 2 (ATP2A2) in the samples is then determined by comparing the O, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, The concentration of ATPase, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain ATPase, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2), ATPase |
| Short name: |
Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA kit, ATPase |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Ca+and Transporting, Ca+and transporting, Cardiac Muscle, Slow Twitch 2 (ATPase, cardiac muscle, slow twitch 2) Enzyme-linked immunosorbent assay reagent, ATPase |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ATP2A2 and IDBG-56943 and ENSG00000174437 and 488, ATP2A2 and IDBG-633099 and ENSBTAG00000001398 and 540568, ATP2B and DAR and DD and SERCA2, Atp2a2 and IDBG-198239 and ENSMUSG00000029467 and 11938, Ca++ transporting, Plasma membranes, calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential, cardiac muscle, slow twitch 2, this GO :0000166 and nucleotide binding and molecular function this GO :0002026 and regulation of the force of heart contraction and biological process this GO :0005388 and calcium-transporting ATPase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006810 and transport and biological process this GO :0006812 and cation transport and biological process this GO :0006816 and calcium ion transport and biological process this GO :0006874 and cellular calcium ion homeostasis and biological process this GO :0006984 and ER-nucleus signaling pathway and biological process this GO :0007155 and cell adhesion and biological process this GO :0007596 and blood coagulation and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008152 and metabolic process and biological process this GO :0008544 and epidermis development and biological process this GO :0010460 and positive regulation of heart rate and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016529 and sarcoplasmic reticulum and cellular component this GO :0019829 and cation-transporting ATPase activity and molecular function this GO :0019899 and enzyme binding and molecular function this GO :0031095 and platelet dense tubular network membrane and cellular component this GO :0033017 and sarcoplasmic reticulum membrane and cellular component this GO :0034220 and ion transmembrane transport and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0044548 and S100 protein binding and molecular function this GO :0045822 and negative regulation of heart contraction and biological process this GO :0046872 and metal ion binding and molecular function this GO :0055085 and transmembrane transport and biological process this GO :0055119 and relaxation of cardiac muscle and biological process this GO :0070296 and sarcoplasmic reticulum calcium ion transport and biological process this GO :0070588 and calcium ion transmembrane transport and biological process this GO :0086036 and regulation of cardiac muscle cell membrane potential and biological process this GO :0086039 and calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential and molecular function, this GO :0000166 : nucleotide binding, this GO :0000166 : nucleotide binding and also this GO :0005388 : calcium-transporting ATPase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008022 : protein C-terminus binding and also this GO :0019829 : cation-transporting ATPase activity and also this GO :0019899 : enzyme binding and also this GO :0044548 : S100 protein binding and also this GO :0046872 : metal ion binding and also this GO :0086039 : calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential, this GO :0005388 : calcium-transporting ATPase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008022 : protein C-terminus binding, this GO :0019829 : cation-transporting ATPase activity, this GO :0019899 : enzyme binding, this GO :0044548 : S100 protein binding, this GO :0046872 : metal ion binding, this GO :0086039 : calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential, ATPase |
| Identity: |
812 |
| Gene: |
ATP2A2 |
More about : ATP2A2 |
| Long gene name: |
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 |
| Synonyms gene: |
ATP2B DAR |
| Synonyms gene name: |
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 |
| Synonyms: |
SERCA2 |
| Synonyms name: |
sarcoplasmic/endoplasmic reticulum calcium ATPase 2 calcium pump 2 |
| Locus: |
12q24, 11 |
| Discovery year: |
1990-09-10 |
| Entrez gene record: |
488 |
| Pubmed identfication: |
10080178 |
| RefSeq identity: |
NM_001681 |
| Classification: |
ATPases Ca2+ transporting |
| Havana BLAST/BLAT: |
OTTHUMG00000169327 |