| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Band 3 / SLC4A1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-F, IHC-P, WB |
| Recommended dilutions: |
1-0, 5-1ug/ml, 5-1ug/ml, 5ug/ml, IHC (Frozen): 0, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Optimal dilution of the Band 3 antibody should be determined by the researcher |
| Intented use: |
This Band 3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P02730 |
| Purity: |
Antigen affinity |
| Description: |
Many mutations in this gene are known in man, Other mutations that do not give rise to disease result in novel blood group antigens, The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin, The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II, The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger, The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions, The protein comprises two domains that are structurally and functionally distinct, The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, This protein is predominantly dimeric but forms tetramers in the presence of ankyrin, and defective kidney acid secretion leading to distal renal tubular acidosis, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs, which form the Diego blood group system, Band 3 is also known as SLC4A1 |
| Immunogen: |
Amino acids 28-365 of human Band 3 were used as the immunogen for the Band 3 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Band 3 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
membrane, Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Band 3 / SLC4A1 |
| Short name: |
Band 3 Antibody / SLC4A1 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Die this GO blood family), anion exchanger, member 1 (erythrocyte membrane protein band 3, Band 3 (Antibody to) / solute carrier family 4 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AE1 and BND3 and CD233 and DI and EMPB3 and EPB3 and FR and RTA1A and SW and WD and WD1 and WR, BB3 and IDBG-640933 and ENSBTAG00000001610 and 286817, Die this GO blood group), Plasma membranes, SLC4A1 and IDBG-53601 and ENSG00000004939 and 6521, Slc4a1 and IDBG-212590 and ENSMUSG00000006574 and 20533, anion exchanger, member 1 (erythrocyte membrane protein band 3, protein anchor, this GO :0003779 and actin binding and molecular function this GO :0005215 and transporter activity and molecular function this GO :0005452 and inorganic anion exchanger activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006810 and transport and biological process this GO :0006811 and ion transport and biological process this GO :0006820 and anion transport and biological process this GO :0006821 and chloride transport and biological process this GO :0006873 and cellular ion homeostasis and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008509 and anion transmembrane transporter activity and molecular function this GO :0015106 and bicarbonate transmembrane transporter activity and molecular function this GO :0015108 and chloride transmembrane transporter activity and molecular function this GO :0015301 and anion:anion antiporter activity and molecular function this GO :0015701 and bicarbonate transport and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016323 and basolateral plasma membrane and cellular component this GO :0030018 and Z disc and cellular component this GO :0030506 and ankyrin binding and molecular function this GO :0030863 and cortical cytoskeleton and cellular component this GO :0042803 and protein homodimerization activity and molecular function this GO :0043495 and protein anchor and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0055085 and transmembrane transport and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0072562 and blood microparticle and cellular component this GO :1902476 and chloride transmembrane transport and biological process, this GO :0003779 : actin binding, this GO :0003779 : actin binding and also this GO :0005215 : transporter activity and also this GO :0005452 : inorganic anion exchanger activity and also this GO :0005515 : protein binding and also this GO :0008022 : protein C-terminus binding and also this GO :0008509 : anion transmembrane transporter activity and also this GO :0015106 : bicarbonate transmembrane transporter activity and also this GO :0015108 : chloride transmembrane transporter activity and also this GO :0015301 : anion:anion antiporter activity and also this GO :0030506 : ankyrin binding and also this GO :0042803 : protein homodimerization activity and also this GO :0043495 : protein anchor, this GO :0005215 : transporter activity, this GO :0005452 : inorganic anion exchanger activity, this GO :0005515 : protein binding, this GO :0008022 : protein C-terminus binding, this GO :0008509 : anion transmembrane transporter activity, this GO :0015106 : bicarbonate transmembrane transporter activity, this GO :0015108 : chloride transmembrane transporter activity, this GO :0015301 : anion:anion antiporter activity, this GO :0030506 : ankyrin binding, this GO :0042803 : protein homodimerization activity, this GO :0043495 : protein anchor, solute carrier family 4 |
| Identity: |
11027 |
| Gene: |
SLC4A1 |
More about : SLC4A1 |
| Long gene name: |
solute carrier family 4 member 1 (Diego blood group) |
| Synonyms gene: |
EPB3 AE1 DI WD |
| Synonyms gene name: |
Diego blood group) solute carrier family 4 (anion exchanger), Waldner blood group erythrocyte membrane protein band 3 Diego blood group solute carrier family 4, anion exchanger, member 1 (Diego blood group) , member 1 (erythrocyte membrane protein band 3, member 1 solute carrier family 4 (anion exchanger) |
| Synonyms: |
RTA1A CD233 FR SW WR |
| Synonyms name: |
Froese blood group Swann blood group Wright blood group |
| Locus: |
17q21, 31 |
| Discovery year: |
1988-04-20 |
| Entrez gene record: |
6521 |
| Pubmed identfication: |
8434259 |
| RefSeq identity: |
NM_000342 |
| Classification: |
Solute carriers CD molecules Blood group antigens |
| Havana BLAST/BLAT: |
OTTHUMG00000156843 |
| Locus Specific Databases: |
Blood Group Antigen Mutation Database LRG_803 |