| Antigen detectable with this ELISA kit: |
Type B (ALOX15B), Arachidonate-15-Lipoxygenase |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
059ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-15-Lipoxygenase, The concentration of Arachidonate-15-Lipoxygenase, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-15-Lipoxygenase, Type B (ALOX15B), Type B (ALOX15B), Type B (ALOX15B), Type B (ALOX15B) in the samples is then determined by comparing the O, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Arachidonate-15-Lipoxygenase, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
B (ALOX15B), Arachidonate-15-Lipoxygenase |
| Short name: |
B (ALOX15B) ELISA kit, Arachidonate-15-Lipoxygenase |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
classification B (arachidonate 15-lipoxygenase, classification B) Enzyme-linked immunosorbent assay reagent, Arachidonate-15-Lipoxygenase |
| Alternative technique: |
elisas |
| Alternative to gene target: |
15-LOX-2, ALOX15B and IDBG-27642 and ENSG00000179593 and 247, ALOX15B and IDBG-635491 and ENSBTAG00000008779 and 286820, Alox8 and IDBG-190116 and ENSMUSG00000020891 and 11688, acting on single donors with incorporation of molecular oxygen, acting on single donors with incorporation of molecular oxygen, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen, incorporation of two atoms of oxygen and also this GO :0036403 : arachidonate 8(S)-lipoxygenase activity and also this GO :0046872 : metal ion binding and also this GO :0050473 : arachidonate 15-lipoxygenase activity, incorporation of two atoms of oxygen and molecular function this GO :0019369 and arachidonic acid metabolic process and biological process this GO :0019372 and lipoxygenase pathway and biological process this GO :0019898 and extrinsic component of membrane and cellular component this GO :0030336 and negative regulation of cell migration and biological process this GO :0030850 and prostate gland development and biological process this GO :0030856 and regulation of epithelial cell differentiation and biological process this GO :0035360 and positive regulation of peroxisome proliferator activated receptor signaling pathway and biological process this GO :0036403 and arachidonate 8(S)-lipoxygenase activity and molecular function this GO :0043651 and linoleic acid metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045618 and positive regulation of keratinocyte differentiation and biological process this GO :0045786 and negative regulation of cell cycle and biological process this GO :0045926 and negative regulation of growth and biological process this GO :0046872 and metal ion binding and molecular function this GO :0050473 and arachidonate 15-lipoxygenase activity and molecular function this GO :0051122 and hepoxilin biosynthetic process and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0090197 and positive regulation of chemokine secretion and biological process, nuclei, oxidoreductase activity, this GO :0005506 and iron ion binding and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006629 and lipid metabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008289 and lipid binding and molecular function this GO :0010744 and positive regulation of macrophage derived foam cell differentiation and biological process this GO :0016020 and membrane and cellular component this GO :0016165 and lipoxygenase activity and molecular function this GO :0016702 and oxidoreductase activity, this GO :0005506 : iron ion binding, this GO :0005506 : iron ion binding and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0008289 : lipid binding and also this GO :0016165 : lipoxygenase activity and also this GO :0016702 : oxidoreductase activity, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, this GO :0008289 : lipid binding, this GO :0016165 : lipoxygenase activity, this GO :0016702 : oxidoreductase activity, this GO :0036403 : arachidonate 8(S)-lipoxygenase activity, this GO :0046872 : metal ion binding, this GO :0050473 : arachidonate 15-lipoxygenase activity, type B, arachidonate 15-lipoxygenase |
| Identity: |
434 |
| Gene: |
ALOX15B |
More about : ALOX15B |
| Long gene name: |
arachidonate 15-lipoxygenase, type B |
| Synonyms gene name: |
arachidonate 15-lipoxygenase, second type |
| Synonyms: |
15-LOX-2 |
| Locus: |
17p13, 1 |
| Discovery year: |
1998-07-22 |
| GenBank acession: |
U78294 |
| Entrez gene record: |
247 |
| Pubmed identfication: |
9177185 |
| Classification: |
Arachidonate lipoxygenases |
| Havana BLAST/BLAT: |
OTTHUMG00000108181 |