| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
IRF8 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the IRF8 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This IRF8 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q02556 |
| Purity: |
Antigen affinity |
| Description: |
By genomic sequence analysis, IRF family proteins also control expression of IFNalpha and IFNbeta-regulated genes that are induced by viral infection, IRF8 is a transcription factor that plays critical roles in the regulation of lineage commitment and in myeloid cell maturation including the decision for a common myeloid progenitor(CMP) to differentiate into a monocyte precursor cell, It is a transcription factor of the interferon regulatory factor (IRF) family, The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, also known as Interferon consensus sequence-binding protein (ICSBP), is a protein that in humans is encoded by the IRF8 gene, namely IFNalpha and IFNbeta, the gene is mapped to chromosome 16q24, 1, Interferon regulatory factor 8 |
| Immunogen: |
An amino acid sequence from the C-terminus of human Interferon regulatory factor 8 (ASHQRSFFRENQQITV) was used as the immunogen for this IRF8 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the IRF8 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
IRF8 |
| Short name: |
IRF8 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
interferon regulatory factor 8 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0006909 and pha this GO cytosis and biological process this GO :0006955 and immune response and biological process this GO :0009617 and response to bacterium and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0030099 and myeloid cell differentiation and biological process this GO :0032729 and positive regulation of interferon-gamma production and biological process this GO :0032735 and positive regulation of interleukin-12 production and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0042832 and defense response to protozoan and biological process this GO :0044130 and negative regulation of growth of symbiont in host and biological process this GO :0045087 and innate immune response and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0060261 and positive regulation of transcription initiation from RNA polymerase II promoter and biological process this GO :0060333 and interferon-gamma-mediated signaling pathway and biological process this GO :0060337 and type I interferon signaling pathway and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process, H-ICSBP and ICSBP and ICSBP1 and IMD32A and IMD32B and IRF-8, IRF8 and IDBG-45278 and ENSG00000140968 and 3394, IRF8 and IDBG-630167 and ENSBTAG00000017824 and 614909, Irf8 and IDBG-196509 and ENSMUSG00000041515 and 15900, nuclei, protein binding, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000975 and regulatory region DNA binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003705 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005829 and cytosol and cellular component this GO :0006355 and regulation of transcription, this GO :0000975 : regulatory region DNA binding, this GO :0000975 : regulatory region DNA binding and also this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding, this GO :0003677 : DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, interferon regulatory factor 8 |
| Identity: |
5358 |
| Gene: |
IRF8 |
More about : IRF8 |
| Long gene name: |
interferon regulatory factor 8 |
| Synonyms gene: |
ICSBP1 |
| Synonyms gene name: |
interferon consensus sequence binding protein 1 |
| Synonyms: |
IRF-8 ICSBP |
| Locus: |
16q24, 1 |
| Discovery year: |
1993-09-09 |
| GenBank acession: |
M91196 |
| Entrez gene record: |
3394 |
| Pubmed identfication: |
1460054 11997525 |
| RefSeq identity: |
NM_002163 |
| Havana BLAST/BLAT: |
OTTHUMG00000137648 |
| Locus Specific Databases: |
LRG_294 |