| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
NKCC1 SLC12A2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-F, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Frozen): 0, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the NKCC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This NKCC1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P55011 |
| Purity: |
Antigen affinity |
| Description: |
By fluorescence in situ hybridization, NKCC1 is phosphorylated in response to DNA damage, The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume, The protein encoded by this gene mediates sodium and chloride transport and reabsorption, Three transcript variants encoding two different isoforms have been found for this gene, also known as NKCC1 (Na-K-Cl Cotransporter 1), called exocrine glands, especially in organs that secrete fluids, is widely distributed throughout the body, member 2, this gene is mapped to chromosome 5q23, 3, Solute carrier family 12 (sodium/potassium/chloride transporters) |
| Immunogen: |
mouse and rat), An amino acid sequence from the N-terminus of human NKCC1 (RIDHYRHTAAQLGEKLLRP) was used as the immunogen for this NKCC1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the NKCC1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
NKCC1 SLC12A2 |
| Short name: |
NKCC1 Antibody SLC12A2 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
NKCC1 (Antibody to) SLC12A2 |
| Alternative technique: |
antibodies |
| Identity: |
10911 |
| Gene: |
SLC12A2 |
More about : SLC12A2 |
| Long gene name: |
solute carrier family 12 member 2 |
| Synonyms gene name: |
member 2 , solute carrier family 12 (sodium/potassium/chloride transporter) |
| Synonyms: |
NKCC1 BSC BSC2 PPP1R141 |
| Synonyms name: |
bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 basolateral Na-K-Cl symporter protein phosphatase 1, regulatory subunit 141 |
| Locus: |
5q23, 3 |
| Discovery year: |
1994-02-16 |
| Entrez gene record: |
6558 |
| Pubmed identfication: |
7629105 |
| RefSeq identity: |
NM_001046 |
| Classification: |
Solute carriers Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000128983 |