| Antigen detectable with this ELISA kit: |
Apolipoprotein B (APOB) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1000ng/mL, 125ng/mL, 250ng/mL, 31, 500ng/mL, 62, 25-2000ng/mL The standard curve concentrations used for the ELISA&rsquo, 25ng/mL, 31, 5ng/mL, s were 2000ng/mL |
| Sensitivity: |
3ng/mL, The minimum detectable dose of this kit is typically less than 14 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein B (APOB), The concentration of Apolipoprotein B (APOB) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein B (APOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Apolipoprotein B (APOB), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Apolipoprotein B (APOB) |
| Short name: |
Apolipoprotein B (APOB) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Apolipoprotein B (apolipoprotein B (including Ag(x) antigen)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
APOB and IDBG-32746 and ENSG00000084674 and 338, APOB and IDBG-637535 and ENSBTAG00000008505 and 494004, Apob and IDBG-128209 and ENSMUSG00000020609 and 238055, Extracellular, FLDB and LDLCQ4, low-density lipoprotein particle receptor binding, this GO :0001523 and retinoid metabolic process and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0005319 and lipid transporter activity and molecular function this GO :0005488 and binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005543 and phospholipid binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005769 and early endosome and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006629 and lipid metabolic process and biological process this GO :0006642 and triglyceride mobilization and biological process this GO :0006869 and lipid transport and biological process this GO :0006898 and receptor-mediated endocytosis and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007399 and nervous system development and biological process this GO :0007596 and blood coagulation and biological process this GO :0007603 and phototransduction, this GO :0005319 : lipid transporter activity, this GO :0005319 : lipid transporter activity and also this GO :0005488 : binding and also this GO :0005515 : protein binding and also this GO :0005543 : phospholipid binding and also this GO :0008201 : heparin binding and also this GO :0008289 : lipid binding and also this GO :0017127 : cholesterol transporter activity and also this GO :0035473 : lipase binding and also this GO :0050750 : low-density lipoprotein particle receptor binding, this GO :0005488 : binding, this GO :0005515 : protein binding, this GO :0005543 : phospholipid binding, this GO :0008201 : heparin binding, this GO :0008289 : lipid binding, this GO :0017127 : cholesterol transporter activity, this GO :0035473 : lipase binding, this GO :0050750 : low-density lipoprotein particle receptor binding, visible light and biological process this GO :0008201 and heparin binding and molecular function this GO :0008203 and cholesterol metabolic process and biological process this GO :0008289 and lipid binding and molecular function this GO :0009566 and fertilization and biological process this GO :0009615 and response to virus and biological process this GO :0009743 and response to carbohydrate and biological process this GO :0009791 and post-embryonic development and biological process this GO :0010008 and endosome membrane and cellular component this GO :0010033 and response to organic substance and biological process this GO :0010269 and response to selenium ion and biological process this GO :0010744 and positive regulation of macrophage derived foam cell differentiation and biological process this GO :0010884 and positive regulation of lipid storage and biological process this GO :0010886 and positive regulation of cholesterol storage and biological process this GO :0012506 and vesicle membrane and cellular component this GO :0015629 and actin cytoskeleton and cellular component this GO :0016042 and lipid catabolic process and biological process this GO :0017127 and cholesterol transporter activity and molecular function this GO :0019433 and triglyceride catabolic process and biological process this GO :0030301 and cholesterol transport and biological process this GO :0030317 and sperm motility and biological process this GO :0030669 and clathrin-coated endocytic vesicle membrane and cellular component this GO :0031904 and endosome lumen and cellular component this GO :0031983 and vesicle lumen and cellular component this GO :0032496 and response to lipopolysaccharide and biological process this GO :0033344 and cholesterol efflux and biological process this GO :0034359 and mature chylomicron and cellular component this GO :0034360 and chylomicron remnant and cellular component this GO :0034361 and very-low-density lipoprotein particle and cellular component this GO :0034362 and low-density lipoprotein particle and cellular component this GO :0034363 and intermediate-density lipoprotein particle and cellular component this GO :0034374 and low-density lipoprotein particle remodeling and biological process this GO :0034379 and very-low-density lipoprotein particle assembly and biological process this GO :0034383 and low-density lipoprotein particle clearance and biological process this GO :0035473 and lipase binding and molecular function this GO :0042157 and lipoprotein metabolic process and biological process this GO :0042158 and lipoprotein biosynthetic process and biological process this GO :0042159 and lipoprotein catabolic process and biological process this GO :0042627 and chylomicron and cellular component this GO :0042632 and cholesterol homeostasis and biological process this GO :0042953 and lipoprotein transport and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0044281 and small molecule metabolic process and biological process this GO :0045540 and regulation of cholesterol biosynthetic process and biological process this GO :0048844 and artery morphogenesis and biological process this GO :0050750 and low-density lipoprotein particle receptor binding and molecular function this GO :0050900 and leukocyte migration and biological process this GO :0071356 and cellular response to tumor necrosis factor and biological process this GO :0071379 and cellular response to prostaglandin stimulus and biological process this GO :0071682 and endocytic vesicle lumen and cellular component, apolipoprotein B (including Ag(x) antigen) |
| Identity: |
603 |
| Gene: |
APOB |
More about : APOB |
| Long gene name: |
apolipoprotein B |
| Synonyms gene name: |
apolipoprotein B (including Ag(x) antigen) |
| Locus: |
2p24, 1 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M14162 |
| Entrez gene record: |
338 |
| Classification: |
Apolipoproteins |
| Havana BLAST/BLAT: |
OTTHUMG00000090785 |