| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PGK1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
IHC-P: 0, 5-1ug/ml, 5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This PGK1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P00558 |
| Purity: |
Antigen affinity |
| Description: |
An exception to this rule is a reductase secreted by tumor cells that reduces disulfide bonds in the serine proteinase plasmin, Disulfide bonds in secreted proteins are considered to be inert because of the oxidizing nature of the extracellular milieu, Lay et al, Plasma of mice bearing fibrosarcoma tumors contained several-fold more phosphoglycerate kinase, Recombinant phosphoglycerate kinase had the same specific activity as the fibrosarcoma-derived protein, Willard et al, also known as PGKA, as compared with mice without tumors, is an enzyme that in humans is encoded by the PGK1 gene, (1985) used a cDNA for human PGK to map the functional PGK1 gene to Xq13, (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase, Phosphoglycerate Kinase 1 |
| Immunogen: |
mouse and rat), An amino acid sequence from the middle region of human Phosphoglycerate Kinase 1 (FGTAHRAHSSMVGVN) was used as the immunogen for this PGK1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PGK1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PGK1 |
| Short name: |
PGK1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PGK1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8896 |
| Gene: |
PGK1 |
More about : PGK1 |
| Long gene name: |
phosphoglycerate kinase 1 |
| Locus: |
Xq21, 1 |
| Discovery year: |
1989-04-24 |
| GenBank acession: |
L00159 |
| Entrez gene record: |
5230 |
| Pubmed identfication: |
6188151 6099325 |
| Havana BLAST/BLAT: |
OTTHUMG00000021888 |
| Locus Specific Databases: |
Mental Retardation database |