| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
TSHR |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the TSHR antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This TSHR antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P16473 |
| Purity: |
Antigen affinity |
| Description: |
The TSH receptor differs from the LHCG receptor by the presence of 2 unique insertions of 8 and 50 amino acids in the extracellular domain, Wadsworth et al, also called TSHR and LGR3 has been mapped to chromosome 12 using linkage studies in interspecies backcross mice, (1990) showed that the 8-amino acid tract near the amino terminus of the receptor is an important site of interaction with both TSH and auto-antibodies against thyroid-stimulating immunoglobulins, Thyroid-Stimulating Hormone Receptor |
| Immunogen: |
An amino acid sequence from the N-terminus of human TSH Receptor (ECHQEEDFRVTCKDIQRI) was used as the immunogen for this TSHR antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the TSHR antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
TSHR |
| Short name: |
TSHR Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
TSHR (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12373 |
| Gene: |
TSHR |
More about : TSHR |
| Long gene name: |
thyroid stimulating hormone receptor |
| Synonyms: |
LGR3 |
| Locus: |
14q24-q31 |
| Discovery year: |
1990-03-05 |
| GenBank acession: |
AY429111 |
| Entrez gene record: |
7253 |
| Pubmed identfication: |
2558651 2610690 |
| RefSeq identity: |
NM_000369 |
| Classification: |
Glycoprotein hormone receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000171429 |
| Locus Specific Databases: |
TSH Receptor mutation database LRG_523 |