| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
VASA (DDX4) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-F, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Frozen): 0, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the VASA antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This VASA antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9NQI0 |
| Purity: |
Antigen affinity |
| Description: |
1997), By fluorescence in situ hybridization, By radiation hybrid analysis, Castrillon et al, Chu et al, The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins, This region is syntenic to the distal end of mouse chromosome 13, Using a combination of proteomics, also known as DDX4 and VASA contains the 8 conserved domains found in all known DEAD box proteins, and functional analysis in C, cytology, elegans, they refined the localization to 5q11, where the mouse homolog resides(Abe and Noce, (2000) mapped the gene to 5q, (2006) reduced 1, 099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis, 2-q12, DEAD/H BOX 4 |
| Immunogen: |
mouse and rat homology), Amino acids 253-272 (EDEDSIFAHYQTGINFDKYD) were used as the immunogen for this VASA antibody (100% human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the VASA antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
VASA (DDX4) |
| Short name: |
VASA Antibody (DDX4) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
VASA (Antibody to) (DDX4) |
| Alternative technique: |
antibodies |
| Identity: |
18700 |
| Gene: |
DDX4 |
More about : DDX4 |
| Long gene name: |
DEAD-box helicase 4 |
| Synonyms gene name: |
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 |
| Synonyms: |
VASA |
| Locus: |
5q11, 2 |
| Discovery year: |
2002-05-30 |
| GenBank acession: |
AF262962 |
| Entrez gene record: |
54514 |
| Pubmed identfication: |
10920202 11850529 |
| RefSeq identity: |
NM_024415 |
| Classification: |
DEAD-box helicases |
| Havana BLAST/BLAT: |
OTTHUMG00000097044 |